四季青中三萜类化学成分的研究

Triterpenoids from leaves of Ilex chinensis

目的研究四季青Ilex chinensis中的三萜类化学成分。方法应用多种色谱分离技术进行分离,通过核磁、质谱等波谱方法鉴定化合物的结构。结果从四季青70%乙醇提取物中分离得到13个三萜类化合物,分别鉴定为3β,23-二羟基乌苏-12-烯-28-酸-28-O-β-D-吡喃葡萄糖酯苷(1)、quadranoside Ⅳ(2)、玉叶金花苷R(3)、monepaloside F(4)、冬青苷XXⅦ(5)、冬青苷XXXⅦ(6)、冬青苷XXXⅧ(7)、冬青苷XLI(8)、niga-ichigoside F1(9)、kalidiumoside D(10)、竹节参苷Ⅳa(11)、长圆果冬青苷M(12)、23-羟基-白桦酸-28-O-β-D-吡喃葡萄糖酯苷(13)。结论化合物1、10、13为首次从冬青属植物中分离得到,化合物2~8、11、12为首次从该植物中分离得到。

Objective To study the triterpenoids from the leaves of Ilex chinensis. Methods The constituents were isolated and purified by various chromatographic methods, and the structures were elucidated by spectroscopic analysis. Results Thirteen triterpenoid saponins were isolated from 70% ethanol extracts in the leaves of I. chinensis and identified as 3β,23-dihydroxyurs-12-en-28-oic acid 28-O-β-D-glucopyranoside（1）, quadranoside Ⅳ（2）, mussaendoside R（3）, monepaloside F（4）, ilexoside XXⅦ（5）, ilexoside XXXⅦ（6）, ilexoside XXXⅧ（7）, ilexoside XLI（8）, niga-ichigoside F1（9）, kalidiumoside D（10）, chikusetsusaponin Ⅳa（11）, oblonganoside M（12）, and 23-hydroxy-betulinic acid-28-O-β-D-glucopyranoside（13）. Conclusion Compounds 1, 10, and 13 are isolated from the genus Ilex for the first time. Compounds 2—8, 11, and 12 are isolated from this plant for the first time.