海带多糖对实验性动脉粥样硬化大鼠血脂和NO及NOS的影响

Effect of Laminaria Polysaccharide on Blood Lipids, Nitric Oxide and Nitric Oxide Synthase in Experimental Atherosclerosis Rats

探讨海带多糖（LJP）对实验性动脉粥样硬化（AS）大鼠血脂和NO及NOS的影响。采用高脂饲料＋维生素D3（VD3）饲养方法建立大鼠动脉粥样硬化模型,12周造模成功后,将大鼠随机分为6组,每组10只,分别为正常组、模型组、辛伐他汀对照组及海带多糖低剂量（250 mg·kg-1·d-1）、中剂量（500 mg·kg-1·d-1）、高剂量（1 000 mg·kg-1·d-1）治疗组。海带多糖治疗组在给药8周后,制备主动脉冰冻切片,HE染色观察主动脉病理组织学变化。颈动脉取血测定血清中总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）的含量;分别测定血清与主动脉中的一氧化氮（NO）和一氧化氮合酶（NOS）水平。结果显示,与模型组比较,HE染色观察发现LJP治疗组能抑制内膜与中膜平滑肌增生,减少主动脉内皮脂质沉积与胆固醇的结晶;与模型组比较,高剂量LJP可明显降低大鼠血清中TC、TG、LDL-C水平,同时LJP也会明显提高HDL-C水平（p〈0.05）,中剂量LJP能够降低大鼠血清中TG水平,同时LJP也会明显提高HDL-C水平（p〈0.05）,高、中剂量的LJP均可提高模型大鼠血清和主动脉中NO和NOS水平（p〈0.05）。LJP可改善动脉粥样硬化（AS）大鼠血脂水平,对AS形成以及发展具有一定的治疗效果,其可能是机制是通过调节血脂水平,提高NO和NOS的水平,稳定AS斑块,从而有效地抑制AS疾病的发生和发展。

To investigate the effects of laminarin（LJP） on blood lipids, nitric oxide and nitric oxide synthase in experimental atherosclerosis rats. Atherosclerosis model was established by high-fat diet＋vitamin D3（VD3） feeding method. After 12 weeks of successful model establishment, the rats were randomly divided into 6 groups of 10 rats each, which were normal group, model group, Simvastatin control group and low dose of kelp polysaccharide（250 mg·kg-1·d-1）, medium dose（500 mg·kg-1·d-1）, high dose（1 000 mg·kg-1·d-1） treatment group. The kelp polysaccharide treatment group 8 d after administration, preparation of aortic frozen sections, HE staining observed aortic pathological changes. The content of total cholesterol（TC）, triglyceride（TG）, high density lipoprotein cholesterol（HDL-C） and low density lipoprotein cholesterol（LDL-C） in the serum were measured by carotid blood sampling. In the levels of nitric oxide（NO） and nitric oxide synthase（NOS）. The results showed that, compared with the model group, HE staining showed that the LJP treatment group could inhibit the proliferation of intima and media smooth muscle and reduce the aortic endothelial lipid deposition and cholesterol crystallization;Compared with the model group, LJP could significantly reduce the rats Serum levels of TC, TG, LDL-C, while LJP also significantly increased HDL-C levels（p〈0.05）, medium dose LJP can reduce serum TG levels in rats, while LJP also significantly increased HDL-C levels（p〈0.05）. Both high and medium doses of LJP increased the levels of NO and NOS in serum and aorta in model rats（p〈0.05）. LJP can improve blood lipid level of atherosclerosis（AS） rats and has certain therapeutic effect on the formation and development of AS. LJP can improve the blood lipid level of atherosclerotic（AS） rats, and has certain therapeutic effect on the formation and development of AS.It may be that the mechanism is to regulate the level of NO and NOS and stabilize AS plaque by regulating the level of blood lipids, so as to effectively inhibit the occurrence and development of AS disease.