不同剂量外用重组人碱性成纤维细胞生长因子对皮肤和肌肉的影响

Effect of different dosage of recombinant human basic fibroblast growth factor on skin and muscle

目的评价重组人碱性成纤维细胞生长因子（rh-bFGF）对皮肤和肌肉的影响。方法选取雌性6周BALB／c小鼠40只，随机分为皮肤组和肌肉组，每组各20只小鼠。其中皮肤组随机分为A实验组（14只）、B对照组（6只）。皮肤组在下颏部分别注射100U rh—bFGF和同等剂量的生理盐水。肌肉组随机分为C、D、E、F4组，每组5只小鼠，肌肉组在左侧腓肠肌依次注射200、400、800U rh-hFGF和同等剂量的生理盐水。在8周时取材。HE染色观察下颏组织和肌纤维形态。马松三色染色检测两组肌纤维、胶原及纤维组织分布。免疫组织化学检测CD34测定下颏和腓肠肌的血管密度。结果第8周时A组有10只小鼠出现下颏部隆起，C～D组腓肠肌均出现肥大。A组表皮、真皮乳头层和黏膜的厚度、毛囊直径、血管数量、胶原及纤维含量均明显大于B组（P〈O．05）。C～E组腓肠肌最宽处的距离和血管数量明显高于F组（P〈0．05）。结论rh—bFGF皮下注射可使下颏皮肤增厚、血管增加、毛囊增粗、胶原及纤维组织增多。rh-bFGF肌内注射可以使肌纤维肥大和血．管增多。

Objective To observe the effect of external recombinant human basic fibroblast growth factor （rh-bFGF） on the skin and muscles. Methods 40 BALB/c mice of 6 weeks were randomly divided into skin and muscle groups. The skin group was randomly divided into group A and group B. The skin group was injected with 100U rh-bFGF and the same dose of saline in the chin. The muscle groups were randomly divided into C, D, E, and F, and the muscle group was injected into the left calf muscle in order of 200, 400, 800U rh-bFGF and the same dose of normal saline. The materal was taken at week 8. HE staining was used to observe the submental tissue and muscle fiber morphology. Two groups of muscle fibers, collagen and fibrous tissue were detected by trichrome stain. Immunohistochemistry was used to determine the blood vessel density of the chin and gastrocnemius. Results In week 8, 10 mice in group A had a mass in the lower jaw, and the gastrocnemius of the groups C and D showed hypertrophy. Group A showed the thickness of epidermis, dermal papillary layer and mucosa, hair follicle and blood vessel quantity, glandular cavity, collagen and fiber content were sigificantly greater than that of group B （P〈0.05）. In the groups C, D and E, the gastrocnemius muscle was significantly thicker than that of group F, and the thickening of group D was the most obvious （P〈0.05）, and the contents of collagen and fiber in the groups C, D and E were significantly higher than that in group F. Conclusions Subcutaneous injection of rh-bFGF leads to submental skin thickening, vascular hyperplasia, increased diameter of hair follicle and higher collagen and fiber contents; intramuscular injection of it may induce the muscular hypertrophy and vascular proliferation.