紫小麦花色苷的提取及其抗氧化和肿瘤抑制研究

Extraction of Anthocyanins from Purple Wheat and Its Effect on Antioxidant Activity and Inhibitory on Tumor Cells

为了研究紫粒小麦花色苷的高效提取方法、抗氧化和肿瘤抑制的作用,以紫粒小麦农大3753的麦麸为原料,采用改良CTAB法提取花色苷,响应面法优化提取工艺;利用DMPD+法测定花色苷抗氧化活性;体外实验研究小麦花色苷对胃癌细胞HGC-27和MGC-803的侵染和增殖的抑制作用。响应面实验结果表明,提取时间1.1 h、料液比为1 g∶3.2 m L、浸提3次提取率最高,为3.40 g/kg;花色苷的抗氧化活性高于水溶性维生素E;花色苷可以抑制胃癌细胞HGC-27和MGC-803的侵染能力和增殖作用。上述结果表明改良CTAB提取法易于操作,提取出的花色苷纯度高,具有较高抗氧化活性和肿瘤抑制作用。

In order to provide an efficient method for extracting anthoeyanins from purple wheat bran and understand its antioxidant and inhibitory effect on tumor cells, this experiment took the bran of purple wheat ＇ Nongda 3753＇ as material, extracted anthoeyanin by the improved CTAB method and response surface methodology to optimize the extraction process. DMPD^＋ method was also used to measure the anthocyaninson antioxidant activity. HGC-27 and MGC-803 were used to determine the effect of anthocyaninson on tumor cells proliferation and invasion. The results indicated that the best process parameters of extraction were solid-liquid ratio 1 g：3.2 mL, extraction time 1.1 h, extration number 3 times ; and the yield was 3.40 g/kg. Antioxidant experiments showed that the antioxidant activity of anthocyanins was higher than that of water soluble vitamin E. Moreover, anthocyanins could inhibit the invasion and proliferation of gastric cancer cells HGC-27 and MGC-803. These results indicated that the improved CTAB extrcation method was easy to operate, and the anthocyanins extracted had higher purity and higher antioxidant activity and tumor inhibition effect.