β-甘露聚糖酶分子生物学研究进展

Research Progress on Molecular Biology of β-mannanases

β-甘露聚糖酶在食品、饲料、造纸、洗涤等工业领域应用广泛。近几年,基因测序技术飞跃发展,研究人员挖掘到许多新颖的β-甘露聚糖酶基因并对其进行了酶学性质的研究。介绍了各种类型的甘露聚糖及其降解酶,梳理了β-甘露聚糖酶的糖苷水解酶家族分类、来源、结构和催化机理,归纳总结了近几年微生物来源β-甘露聚糖酶的重组表达、酶学性质及分子改造,简述了甘露聚糖酶在食品和饲料等方面的应用,展望了β-甘露聚糖酶的研究热点及方向。

Endo-β-mannanase is widely applied in various industries including food, feed, paper making and lavation. Recently, with the overflying development of gene sequencing technology, many novel endo-β-mannanases have been unearthed and their enzymatic properties have been investigated in recent year. The paper introduced various types of mannan and its degrading enzymes ; summarized classification, sources, structures and catalytic mechanisms of endo- β-mannanases; and summarized the recombination expression, enzymatic properties and molecular modification of endo-β-mannanases sourced from microorganisms in recent years. Furthermore, the paper briefly described the application of endo-β-mannanases in food and feed industries and prospected the research hotspot and direction of endo-β-mannanase.