中国侵袭性肺炎链球菌19A、19F血清型的基因分型分析

Genotyping of invasive Streptococcus pneumoniae serotype 19A and 19F in China

目的研究中国侵袭性肺炎链球菌19A、19F血清型菌株的基因型特征。方法采用多位点序列分型(MLST)法,对来自全国各疾病预防控制中心细菌性疫苗可预防疾病监测网络实验室的57株侵袭性肺炎链球菌进行ST序列分型,利用Bio Numerics软件处理分型数据并进行ST型聚类分析。结果 35株侵袭性肺炎链球菌19A血清型菌株共分为6个ST型,其中ST320型29株,占82.9%,为优势基因型。其他6株分别为ST876(2株,5.7%)、ST4560(1株,2.9%)、ST9230(1株,2.9%)、ST7759(1株,2.9%)和ST6429(1株,2.9%)。22株侵袭性肺炎链球菌19F血清型菌株包括7个ST型,其中ST271型14株,占64%,为优势基因型。其余8株分别为ST236(1株,4.5%)、ST320(2株,9.1%)、ST1937(2株,9.1%)、ST6993(1株,4.5%)、ST9236(1株,4.5%)和ST9240(1株,4.5%)。结论我国侵袭性肺炎链球菌19A血清型菌株主要基因型为ST320型,19F血清型菌株主要基因型为ST271型,ST320型为19A和19F菌株共有的基因型。

Objective To investigate the genotypes of invasive Streptococcus pneumoniae serotype 19A and 19F in China. Methods A total of 57 invasive S. pneumoniae isolates, including 35 serotype 19A strains and 22 serotype 19F strains, were collected through laboratory-based bacterial vaccine preventable disease surveillance network of China. All the isolates were serotyped quellung and PCR. Multilocus sequence typing （MLST） was used for genotyping. Cluster analysis was performed by using software BioNumerics. Results There were 6 STs among 35 invasive S. pneumoniae serotype 19A strains. The predominant sequence type was ST320 （n = 29, 82.9%）, followed by ST876 （n = 2, 5.7%）, ST4560 （n - 1 2.9%）, ST9230 （n = 1, 2.9%） , ST7759 （n = 1, 2.9%） and ST6429 （n= l, 2.9%）. There were 7 STs among 22 invasive S. pneurnoniae serotype 19F strains, including ST271 （n= 14, 63.6%）, ST236 （n= 1,4.5%）, ST320 （n=2 9.1%）, ST1937 （n=2, 9.1%）, ST6993 （n= l, 4.5%） , ST9236 （n= l, 4.5%） and ST9240 （n= 1, 4.5%）. The predominant sequence type was ST271. Conclusion ST320 was the predominant genotype of invasive S. pneumoniae serotype 19A strains. The predominant genotype of 19F invasive strains was ST271. Both the serotype 19A strains and serotype 19F strains had same genotype ST320.