胰岛素样生长因子结合蛋白7对结直肠癌生物学特性的作用

Effects of insulin-like growth factor binding protein 7 gene on the biological characteristics of colorectal cancer

目的:探讨胰岛素样生长因子结合蛋白7(IGFBP7)对结直肠癌生物学特性的作用。方法:培养人结直肠癌SW480细胞,根据转染物不同分为IGFBP7质粒组、空白质粒组和对照组,RT-PCR法检测细胞中IGFBP7基因表达,MTT法检测细胞增殖能力,流式细胞计量术检测细胞凋亡率,Transwell小室检测细胞迁移和侵袭情况。结果:IGFBP7质粒组细胞中IGFBP7mRNA相对表达量高于空白质粒组和对照组,差异有统计学意义(P<0.05);与空白质粒组和对照组相比,IGFBP7质粒组细胞24、48、72和96 h吸光度值均降低,差异有统计学意义(P<0.05);与空白质粒组和对照组相比,IGFBP7质粒组细胞凋亡率升高,差异有统计学意义(P<0.05);IGFBP7质粒组迁移细胞数和侵袭细胞数均低于空白质粒组和对照组,差异均有统计学意义(P<0.05)。结论:IGFBP7基因过表达可抑制结直肠癌细胞增殖、加速细胞凋亡、减少细胞迁移和侵袭。

Objective： To investigate the effects of insulin-like growth factor binding protein 7 gene on the biological characteristics of colorectal cancer. Methods： human colorectal cancer SW480 cells were cultured. According to the different transfectants, the cells were divided into IGF- BP7 plasmid group, blank plasmid group and control group. The expression of IGFBP7 gene was de- tected by RT-PCR. MTT assay was used to detect cell proliferation. Flow cytometry was used to detect the apoptotic rate. Transwell chamber was used to detect cell migration and cell invasion. Results： The relative expression levels of IGFBP7 mRNA in IGFBP7 plasmid group was higher than which in the blank plasmid group and the control group, the difference was statistically significant （P〈0.05）. Compared with the blank plasmid group and the control group, the A values at 24h, 48h, 72h and 96h in the IGFBP7 plasmid group were decreased, the differences were statistically significant（P〈0.05）. Compared with the blank plasmid group and the control group, the apoptosis rate in the IGFBP7 plasmid group was increased, the difference was statistically significant （P〈0.05）. The number of migrating cells and the number of invasive cells in IGFBP7 plasmid group were lower than those in the blank plasmid group and the control group, the differences were statistically significant （P〈0.05）. Concl us ion： Overexpression of I GFBP7 gene could inhibit proliferation of colorectal cancer cells, accelerate cell apoptosis, reduce cell migration and cell invasion.