不同种类B族维生素降低尿酸和保护血管内皮作用探讨

Effect of vitamins B in reducing serum uric acid level and protecting vessel endothelium

目的对比分析各类B族维生素对高尿酸血症(HUA)动物模型血清尿酸水平的降低作用和血管内皮功能的保护作用。方法选择SPF级雄性昆明种小鼠77只,按体质量随机分为6组,对照组12只,别嘌呤醇组13只,维生素B_2组13只,维生素B_6组13只,维生素B_(12)组13只,叶酸组13只。测定各组小鼠血清尿酸和NO水平;免疫组织化学法检测NF-κB和血管内皮细胞黏附因子1(VCAM-1)蛋白的表达。结果别嘌呤醇组、维生素B_2组、维生素B_6组、维生素B_(12)组及叶酸组血清尿酸水平及NF-κB和VCAM-1表达强度明显低于对照组,血清NO水平明显高于对照组(P<0.01)。与别嘌呤醇组比较,维生素B_2组尿酸水平明显升高,NO水平明显降低,而维生素B_(12)组NO水平明显升高(P<0.01)。各组小鼠主动脉内膜形态和血管壁结构未发生改变。对照组主动脉内膜NF-κB和VCAM-1可见连续阳性表达,其他各组表达均有不同程度的减弱。维生素B_2组NF-κB和VCAM-1表达强度明显高于别嘌呤醇组,维生素B_6组、维生素B_(12)组及叶酸组NF-κB和VCAM-1表达强度明显低于别嘌呤醇组(P<0.05,P<0.01)。结论各类B族维生素均可有效降低HUA小鼠血清尿酸水平,同时显著提升血清NO水平。各类B族维生素均可不同程度地抑制HUA小鼠主动脉内膜NF-κB和VCAM-1表达。

Objective To analyze the effect of different kinds of vitamins B in reducing serum UA level and protecting vessel endothelial function in hyperuricemia mouse model.Methods Seventyseven Kunming mice were divided into control group（n=12）,allopurinol group（n=13）,VB2 group（n=13）,VB6 group（n=13）,VB（12） group（n=13）,FA group（n=13）according to their BMI.Serum UA and NO levels were measured,and expressions of NF-κB and VCAM-1 were detected with immunohistochemical staining.Results The serum UA level was significantly lower,the expression levels of NF-κB and VCAM-1 were significantly lower while the serum NO level was significantly higher in allopurinol group,VB groups and FA group than in control group（P〈0.01）.The serum UA level was significantly higher while the serum NO level was significantly lower in VB2 group than in allopurinol group（P〈0.01）.The serum NO level group was significantly higher in VB（12） than in allopurinol group（P〈0.01）.The positive expression levels of NF-κB and VCAM-1 in aortic endothelium were higher in control group than in other groups,significantly higher in VB2 group than in allopurinol group and significantly lower in VB6 group,VB（12） group and FA group than in allopurinol group（P〈0.05,P〈0.01）.Conclusion Different kinds of vitamins B can effectively reduce the serum UA level,increase the serum NO level,and inhibit the expressions of NF-κB and VCAM-1 in hyperuricemia mice.