等毒性剂量芥子气诱导大鼠肺损伤炎性因子和蛋白表达变化

Changes in inflammatory factors and protein expression in sulfur mustard(1LD_(50))-induced pulmonary injury in rats

目的建立经腹腔和气管等毒性剂量(1LD_(50))芥子气(sulfur mustard,SM)诱导大鼠急性肺损伤模型,比较2种模型血清炎性因子水平和肺泡间隔相关蛋白表达的差异。方法选取Sprague Dawley大鼠,随机分为5组,采用ELISA和免疫组化法进行检测。结果 (1)腹腔SM组各时间段血清INF-γ、IL-23水平分别与气管SM组比较显著升高;血清IL-4水平腹腔SM组与气管SM组比较,差异有统计学意义。(2)腹腔SM组大鼠肺泡间隔各时间的NF-Kβ1,NF-Kβp65,ERK,JNK,p38MAPK蛋白阳性表达率分别与气管SM组比较显著升高。结论 SM(1LD50)诱导大鼠急性肺损伤,经腹腔注射血清炎性因子水平和肺泡间隔相关蛋白表达均明显高于经气管灌注,提示SM诱导的肺炎性反应与染毒途径有关。

Objective To establish an animal model of SM by equal toxicity dose（1 LD50）-induced acutepulmonary injury in rats and compare the differences of inflammatory factor and protein expression. Methods Rats were randomly divided into five groups. ELISA and immunohistochemical methods were measured. Results Serum INF-γ and IL-23 levels in the intraperitoneal SM group were increased compared with the tracheal SMgroup;there were also significant differences in serum IL-4 levels between the two groups. In the alveolar septum,the positive expression ratios of NF-Kβ1,NF-Kβp65,ERK,JNK,and p38 MAPK in the intraperitoneal SM groupwere increased compared with the tracheal SM group. Conclusion Using SM（1 LD50）,There are significantlyhigher serum inflammatory factor levels and protein-related expression in the alveolar septum of rats intraperitoneal-ly injected with SM compared with those administered SM by intratracheal instillation. The results suggest that pul-monary inflammatory reactions associated with SM are dependent on the route of exposure.