不同训练状态大鼠心肌GRP78、GRP94和ERP72的表达

GRP78, GRP94 and ERP72 Expressions in Rat Myocardium of Different Training Status

探讨运动诱发心肌适应过程中是否有心肌内质网应激的参与。方法:大鼠进行跑台耐力训练,建立不同训练强度、不同训练时间和周期的大鼠运动模型,在末次训练结束后24 h取材,取左室心肌,采用western blot检测内质网应激相关蛋白GRP78、GRP94、ERP72和CHOP的表达。结果:1)5周运动模型组:时间递增组大鼠心肌GRP78较安静组显著升高,GRP94、ERP72、CHOP蛋白表达无显著性差异;恒时恒速组、速度递增组大鼠心肌GRP78、GRP94、ERP72、CHOP蛋白表达较安静组未见显著性差异。2)10周运动模型:恒时恒速组、速度递增组大鼠心肌GRP78、GRP94、ERP72、CHOP蛋白表达较安静组无显著差异。结论:以延长运动持续时间为特征的5周耐力训练诱发GRP78蛋白表达升高,而速度恒定或速度递增的耐力训练没有诱发心肌与内质网应激相关的蛋白表达,提示运动方式可能与心肌内质网应激有关。

Objective： To explore whether endoplasmic reticulum stress is involved in the process of exercise-induced myocardial adaptation. Methods： Rats were subjected to treadmill exercise training with different exercise intensity, duration and exercise program, and then euthanized 24 h after last run. GRP78,GRP94 and ERP72 expressions in myocardium of left ventricular were measured with western blot analysis.Results： 1） there was a significant increase in the GRP78 expression but no significant difference in the expression of GRP94, ERP72, CHOP in duration-increase group. No significant changes in the expression of GRP78, GRP94, ERP72 and CHOP protein expressions in rats undergoing constant speed and speed-increase training were observed in all rats of 5-week trained groups; 2） No significant changes in the expression of GRP78, GRP94, ERP72 and CHOP were observed in all rats of 10-week trained groups when compared with age-matched control group. Conclusion： GRP78 expression was induced in rat myocardium after five-week duration-increase endurance training, while no significant change in GRP78 expression was observed after constant speed or speed-increase training. It suggests that exercise mode might be associated with exercise-induced endoplasmic reticulum stress.