hMTERF3基因在卵巢癌组织的表达及其对预后的影响

Expression of human MTERF3 gene in ovarian carcinoma and its affection to prognosis:Integration and analysis of the data from Oncomine database

目的探究人线粒体转录终止因子3(human mitochondrial transcription termination factor 3,hMTERF3)在正常卵巢上皮组织和卵巢癌组织中的表达水平及其与卵巢癌患者预后的关系。方法利用Oncomine基因芯片数据库中关于hMTERF3的信息,对比分析正常卵巢上皮组织和卵巢癌组织中hMTERF3基因转录水平的表达数据;提取30例配对卵巢癌组织和正常卵巢上皮组织总蛋白质,Western blot检测hMTERF3蛋白在各组织中的表达水平;利用Kaplan-Meier Plotter数据库分析hMTERF3表达对卵巢癌患者预后的影响。结果 Oncomine数据库中共收集了756个不同类型的研究结果,其中关于hMTERF3表达有统计学差异的研究结果有104个,hMTERF3表达增高的研究有76个,表达降低的研究仅有28个。共有11项研究涉及hMTERF3在卵巢癌组织和正常卵巢上皮组织中的表达,包括995例临床样本,与对照组相比,hMTERF3在卵巢癌组织中高表达,且差异具有统计学意义(P=0.020)。提取30对卵巢癌及其配对的正常卵巢上皮组织总蛋白质,Western blot检测发现,其中28例(93.33%)卵巢癌组织中hMTERF3蛋白表达水平显著增高(P<0.05)。Kaplan-Meier Plotter数据库分析发现,hMTERF3的转录水平表达量与卵巢癌患者生存期存在显著相关性,无论是无进展生存期(PFS)或总生存期(OS),hMTERF3 m RNA表达量越高,患者的总体生存期越差(HR>1.0,P<0.05)。结论通过整合Oncomine基因芯片数据和临床试验检测数据,提示hMTERF3基因在卵巢癌组织中高表达,且与卵巢癌患者的预后不良具有显著的相关性,hMTERF3基因有可能成为卵巢癌预后判断和抗肿瘤药物开发的潜在靶点。

Objective To illustrate the expression and prognostic roles of human mitochondrial transcription termination factor 3（h MTERF3） in ovarian cancer. Methods h MTERF3 expression in ovarian cancer was retrieved from Oncomine database. The protein expression level of h MTERF3 in 30 pairs of ovarian cancer tissues and corresponding normal tissues was detected by Western blot. The prognostic roles of h MTERF3 in ovarian cancer patients were analyzed by using Kaplan-Meier Plotter. Results Totally,156 studies of different types relevant the expression of h MTERF3 were indentified in the oncomine database. The h MTERF3 expression was statistically significant in 104 of the studies,over-expressed in 76 and under-expressed in the other 28. Eleven of the studies,with 995 samples,the expression of h MTERF3 in and ovarian cancer tissues,which was significantly higher than that in the normal tissues（P=0.002）. The experimental results showed that protein expression levels in 28 ovarian cancer tissues（93.33%） of 30 specimens were higher than the adjacent non-cancerous tissues detected by Western blot. Significant correlation was found between the expression in transcript level and survival time（P〈0.05）. High h MTERF3 expression could improve the survival time of the patients with ovarian cancer. Conclusion h MTERF3 is highly expressed in ovarian cancer,and its expression is associated with the prognosis of ovarian cancer,which may be an important target of medication for ovarian cancer.