Mg^(2+)对米曲霉HMP-F28所产α-CPA及β-CPA的调控作用

Effect of Mg^(2+) on the Production of α-CPA, β-CPA in Aspergillus oryzae HMP-F28

在米曲霉HMP-F28所产CPA(环匹阿尼酸)类化合物中,β-CPA是α-CPA的生物合成前体化合物,因此β-CPA的含量很低,而α-CPA是主要代谢产物。在研究HMP-F28所产CPA类代谢产物的调控过程中,采用SPE-LC-MS方法建立了一个对HMP-F28所产CPA类代谢物的快速定性分析方法。在此基础上,发现不同浓度的Mg^(2+)可调控α-CPA和β-CPA相对含量,高浓度Mg^(2+)(320 mmol/L)可使菌株显著提高生物合成中间体β-CPA的产量。由于β-CPA在结构上较α-CPA与IAA拮抗剂hypaphorine更类似,因此本研究为进一步研究其作为IAA拮抗剂诱导植物免疫反应的活性提供参考。

Among CPAs（cyclopiazonic acids） produced by Aspergillus oryzae HMP-F28,β-CPA is a biosynthetic precursor of α-CPA,in consequence the content of β-CPA is very low,and α-CPA is the major metabolite. During the regulation and control process of CPAs production by HMP-F28,a SPE-LC-MS method was adopted to establish a rapid qualitative analysis for CPAs metabolite produced by HMP-F28. It was found based on these that different concentration of Mg^2＋could regulate and control the relative content of α-CPA and β-CPA,high concentration of Mg^2＋（320 mmol/L） could significantly increase the output of β-CPA. Since the structure of β-CPA is more similar to IAA antagonist hypaphorin,therefore,this study has laid a foundation to further study on β-CPA as IAA antagonist to induce the activity of plant immune responses.