下肢动脉粥样硬化性疾病患者血清总同型半胱氨酸和T淋巴细胞亚群的相关性研究

The correlation of serum total homocysteine and T lymphocyte subsets in patients with lower extremity atherosclerosis

目的探讨下肢动脉粥样硬化性疾病(LEAD)患者血清总同型半胱氨酸和T淋巴细胞亚群的相关性。方法选择下肢动脉粥样硬化性疾病患者96例(LEAD组)以及下肢动脉正常的其他疾病患者100例(对照组)为研究对象,检查比较两组患者的血清同型半胱氨酸(Hcy)水平及T淋巴细胞亚群,分析LEAD患者血清Hcy水平与T淋巴细胞亚群的相关性。结果 LEAD组患者的Hcy、CD3+T细胞、CD3+CD4+T细胞、CD3+CD4+/CD3+CD8+比值均明显高于对照组(P<0.01),而CD3+CD8+T细胞显著低于对照组(P<0.05)。相关分析显示,LEAD患者血清Hcy水平与血CD3+、CD3+CD4+以及CD3+CD4+/CD3+CD8+呈正相关(r=0.356,0.568,P均<0.01),与血CD3+CD4+/CD3+CD8+呈正相关(r=0.526,P<0.01)。结论 LEAD患者存在免疫功能的紊乱,其CD4+T细胞异常与LEAD的发病有关,Hcy可通过影响CD4+细胞进而影响动脉粥样硬化的发生。

Objective To investigate the correlation of serum total homocysteine and T lymphocyte subsets in patients with lower extremity atherosclerosis. Methods 96 patients（ group LEAD） with lower extremity atherosclerotic disease（ LEAD） and100 patients with other diseases of lower limb artery（ control group） were included in the study. The serum homocysteine（ Hcy）level and T lymphocyte subsets in the two groups were examined and compared. The correlation between serum Hcy level and T lymphocyte subsets in LEAD patients was analyzed. Results The Hcy,CD3 ＋ T cells,CD3 ＋ CD4 ＋ T cells and CD3 ＋ CD4 ＋/CD3 ＋ CD8 ＋ ratio in group LEAD were significantly higher than those in the control group（ P〈0. 01）. The CD3 ＋ CD8 ＋ T cells were significantly lower than those of the control group（ P〈0. 05）. The correlation analysis shows that,the level of serum Hcy in LEAD patients was positively correlated with CD3 ＋,CD3 ＋ CD4 ＋ and CD3 ＋ CD4 ＋/CD3 ＋ CD8 ＋（ R = 0. 356,0. 568,P〈0. 01）. There is a positive correlation with the blood CD3 ＋ CD4 ＋/CD3 ＋ CD8 ＋（ R = 0. 526, P〈0. 01）.Conclusion LEAD patients have a disorder of immune function,and their CD4 ＋ T cell abnormalities are associated with the onset of LEAD. Hcy can affect the occurrence of atherosclerosis by affecting CD4 ＋ cells.