Cu^(2+)对多粘类芽孢杆菌增殖及其转化人参皂苷的影响

Effects of Cu^(2+) on Paenibacillus polymyxa Proliferation and Conversion of Ginsenosides

目的:研究Cu^(2+)对多粘类芽孢杆菌增殖及转化人参皂苷的影响。方法:采用酶标仪和高效液相色谱仪分别测定加入Cu^(2+)后多粘类芽孢杆菌的增殖及转化人参皂苷情况。结果:Cu^(2+)促进多粘类芽孢杆菌增殖适宜质量浓度为10~50 mg/L,Cu^(2+)质量浓度为30 mg/L时促进多粘类芽孢杆菌增殖效果最好,增殖时间最短,与对照组相比提前1 d,生物量显著增加;转化体系中Cu^(2+)质量浓度为10~50 mg/L时,促进多粘类芽孢杆菌转化人参皂苷,Cu^(2+)质量浓度为30 mg/L时,多粘类芽孢杆菌转化人参皂苷能力最强;但Cu^(2+)质量浓度超过100 mg/L时反而抑制多粘类芽孢杆菌转化人参皂苷,并且随Cu^(2+)质量浓度增大而抑制作用增强。结论:Cu^(2+)质量浓度为30 mg/L时,可促进多粘类芽孢杆菌增殖及其转化人参皂苷。

Objective： This study aimed to examine the effect of Cu2＋ on the proliferation of Paenibacillus polymyxa and the conversion of ginsenosides. Methods： A microplate reader and HPLC were used for this study. Results： Cu2＋ at 10–50 mg/L improved P. polymyxa proliferation and the best effect was observed at 30 mg/L, where it entered the logarithmic growth phase earlier 1 d and the biomass was significantly increased as compared with the control group. The presence of Cu2＋ at 10–50 mg/L in the culture medium improved the conversion of ginsenosides by P. polymyxa and its effect was strongest at 30 mg/L. However, when the concentration of Cu2＋ exceeded 100 mg/L, the conversion of ginsenosides was inhibited, and this inhibition was increased with increasing Cu2＋ concentration. Conclusion： A Cu2＋ concentration of 30 mg/L improved P. polymyxa proliferation and the conversion of ginsenosides.