免核酸提取法与煮沸裂解法对HBV-DNA提取后荧光定量PCR检测结果的影响比较

Comparison of the effects of HBV-DNA extraction and boiling cracking on the results of fluorescence quantitative PCR after HBV-DNA extraction

目的:探究在检测乙型肝炎时,比较免核酸提取法和煮沸裂解法对乙型肝炎病毒DNA提取及使用荧光定量PCR检测结果的影响。方法:选取2015年6月至2017年3月在乙肝防治示范区医院就诊的156例慢性乙型肝炎患者,随机分为对照组(煮沸裂解法)和观察组(免核酸提取法),检测两组的检出率,并分析重复性与相关性。结果:通过对比,观察组的检出率(89.74%,70/78)与对照组(71.79%,56/78)相较,显著升高,差异明显(P<0.05);比较两组的相关性,相关性良好(线性相关,r=0.992);比较两组的重复性,观察组的重复性更好。结论:针对乙型肝炎病毒核酸的提取,免核酸提取法的检出率较高,准确性较高,重复性好,结果稳定,对乙型肝炎病毒的诊断和治疗有很高的临床价值。

Objective： To explore the detection of hepatitis B.To compare the effects of nucleic acid extraction and boiling cleavage on the DNA extraction of hepatitis B virus and the results of fluorescence quantitative PCR detection.Methods： From June 2015 to March 2017, 156 patients with chronic hepatitis B were selected. In the control group （boiling lytic method） and the experimental group （nucleic acid extraction method）, the detection rate of the two groups was detected, and the repeatability and correlation were analyzed. Results： By comparison, the detection rate of the test group was 89.74.Compared with the control group（71.79/56/78）,70 / 78 was significantly higher than that of the control group （71.79/56/78）.Conclusion：The detection rate of nucleic acid of hepatitis B virus is high,the accuracy is high, the repeatability is good, the result is stable , and the diagnosis and treatment of hepatitis B virus have very high clinical value.