苯并(a)芘对细胞模型影响的初步研究

Preliminary Study on the Effect of Benzo(a)pyrene on Cell Models

苯并(a)芘[Benzo(a)pyrene,BaP]是一种性质稳定的强烈致癌物质,一般产生于植物油的生产过程中。本文通过模拟BaP对细胞的毒性作用,构建了PC-12神经细胞、RAW 264.7巨噬细胞和RBL-2H3肥大细胞3种细胞模型,探究BaP对3种不同类型细胞的影响,筛选对于BaP更灵敏的特定细胞株。结果表明,在不同浓度Ba P的作用下,3种细胞的活性均有不同程度的下降。此外,Ca^(2+)水平与PC-12神经细胞的活性具有一定的相关性,分析后认为PC-12神经细胞对BaP的识别更为灵敏。

Benzo（a）pyrene （BaP） is a class of stably strong carcinogen, which is generally produced during the production of vegetable oils. In this paper, three cell models of PC-12 nerve cells, RAW 264.7 macrophages and RBL-2H3 mast cells were constructed to study the effect of BaP on three different types of cells by simulating the toxic effects of BaP on cells, the cell strain with sensitive identification of BaP was screened. The results demonstrates that the cell activeness of PC-12、RAW 264.7 and RBL-2H3 decreased with different concentrations of BaP. Moreover, the level of Ca2＋ have a certain correlation with the activity of PC-12 nerve cells after the stimulation of BaP, PC-12 nerve cells are more sensitive to the identification of BaP after analysis.