辛伐他汀促进大鼠脊髓损伤的修复

Simvastatin promotes repair of spinal cord injury in rats

目的探讨辛伐他汀对大鼠脊髓损伤(SCI)模型修复的影响。方法取健康雌性SD大鼠60只,随机均分为假手术组、SCI组、辛伐他汀组。在SCI建模后的第14天采用Western blot法检测各组大鼠受损脊髓脑源性神经营养因子(BDNF)的表达水平。在SCI模型建成后的第49天进行脊髓组织学检测,评估各组大鼠脊髓的空泡和空洞情况。采用BBB评分标准评价各组大鼠处理后不同时间点的后肢运动功能。结果与SCI组相比,辛伐他汀组BDNF表达明显上调(P<0.05)。与SCI组相比,辛伐他汀组的脊髓空洞和空泡面积明显较少(P<0.05);BBB评分显示相对于SCI组,辛伐他汀组的大鼠后肢运动功能改善更加明显(P<0.05)。结论辛伐他汀可通过上调SCI部位组织的BDNF表达来改善损伤部位微环境,促进损伤部位神经元的存活和再生,从而显著加速SCI后大鼠后肢功能的恢复。这表明辛伐他汀治疗可能是促进SCI修复的理想方法,其可能与促进局部BDNF分泌有关。

Objective To explore the effect of simvastatin on improving spinal cord injury（ SCI） in the rat model.Methods In this study,60 healthy female SD rats were randomly divided into three groups： sham,SCI,simvastatin. The expression levels of brain-derived neurotrophic factor（ BDNF） in the injured spinal cord of each group of rats were measured on day 14 after SCI by Western blot. Spinal cord histology was performed on the 49 th day after spinal cord injury to evaluate the vacuoles and voids of the spinal cord in each group. The hindlimb motor function of each group of rats was evaluated at different time points after post-treatment by BBB test. Results The results showed that BDNF expression was significantly up-regulated in the simvastatin group compared to the SCI group（ P 〈0. 05）. Compared with the SCI group,the cavities and vacuole area of the syringes in the simvastatin group were significantly lower（ P 〈0. 05）. The BBB score showed that the improvement of hindlimb motor function of simvastatin group was more obvious than that of the SCI group（ P 〈0. 05）. Conclusion Simvastatin can improve the survival and regeneration of neurons in the injured site by up-regulating the expression of BDNF in the spinal cord injury site,which can accelerate the recovery of hind limb function in SCI rats. These results clearly suggest that simvastatin therapy may be an ideal treatment for SCI and may be associated with the promotion of local neurotrophic factor secretion.