C8gpm11抗菌肽对口内常见致病菌的作用研究

Effect of antibacterial peptide C8gpm11 on oral common pathogenic bacteria

目的研究新型抗菌肽C8gpm11对口内常见致病菌作用及机制。方法通过测定C8gpm11对口腔常见菌群的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)、部分抑菌浓度(FIC)、部分杀菌浓度(FBC),以及测定唾液环境下C8gpm11的抑菌圈直径,分析其在口腔环境中的杀菌能力,利用扫描电镜观察细菌细胞膜形态。结果 C8gpm11对口内常见致病菌的抗菌作用呈现一定的浓度依赖性,唾液中C8gpm11活性略有影响。扫描电镜观察实验组细菌细胞膜表面有孔洞,细胞皱缩。C8gpm11与CHX联合使用具有协同抗菌作用。结论 C8gpm11具有针对口内常见致病菌的抑菌杀菌作用,为其临床应用提供了基础。

Objective To study the antibacterial activity and mechanism of the novel antimicrobial peptide C8 gpm11 against oral common pathogenic microorganisms. Methods The microbicidal activity of C8 gpm11 was analyzed by MIC,MBC,FIC,FBC,and the bacteriostatic ring diameter of C8 gpm11 in the saliva environment.Scanning electron microscopy（ SEM） was used to observe the bacterial membrane. Results C8 gpm11 showed the microbicidal activity with a concentration-dependent manner. The bacteriostatic ability of C8 gpm11 in oral salivaryenvironment was not weakened obviously. C8 gpm11 in combination with CHX had a synergistic antimicrobial effect. Conclusion C8 gpm11 has a bactericidal effect against oral common pathogenic microorganisms,and provides a basis for its clinical value.