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苯并噻二唑处理对柑橘果实采后病害控制效果及机制分析 被引量:9

Control and Mechanism of Postharvest Diseases in Citrus Fruits by Benzothiadiazole Treatment
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摘要 以‘丰脐’柑橘果实为试材,研究了不同浓度苯并噻二唑(benzothiadiazole,BTH)和青霉菌(Penicillium italicum)、绿霉菌(Penicillium digitatum)、炭疽菌(Colletotrichum gloeosporides)同孔、异孔接种对柑橘果实青霉病、绿霉病、炭疽病的控制效果,筛选出针对这3种病害控制效果最适宜的BTH浓度,在此浓度下考察打孔和浸泡处理对果实果皮内源水杨酸含量和抗病性相关酶活力的影响。实验结果表明:0.25、0.50、1.00 mmol/L 3种浓度的BTH与P.italicum、P.digitatum、C.gloeosporioides同孔或异孔接种均可以降低果实青霉病、绿霉病、炭疽病的病斑直径,其中,0.50 mmol/L的BTH效果最好;在病害发病率方面,3种浓度的BTH处理均在果实发病初期有效果,后期效果不显著,相比之下,0.50 mmol/L的BTH效果较好。最适浓度下,通过对果实果皮内源水杨酸含量和抗病性相关酶活力的研究发现,BTH损伤接种能增加果皮内源水杨酸的含量,BTH浸泡处理对果皮内源游离态水杨酸含量无显著影响,但能促进果皮内源结合态水杨酸含量的积累;BTH损伤接种和浸泡处理均能增加柑橘果皮β-1,3葡聚糖酶、几丁质酶、苯丙氨酸解氨酶、过氧化物酶和多酚氧化酶的活力。由此推测,BTH处理诱导柑橘果实提高了其采后抗病性。 (Fengqi Navel) orange fruits (Citrus sinensis L. Osbeck) were used as experimental subjects to evaluate the effcacy of different concentrations of benzothiadiazole (BTH) in controlling lesion diameters and disease incidence in citrus fruits inoculated with Penicillium italicum, Penicillium digitatum and Colletotrichum gloeosporides separately in the same and different wounds. At the same time, the optimum concentration of BTH to control these diseases was determined. The effects of BTH wound inoculation and dipping treatment on endogenous salicylic acid (SA) content and disease resistance-related enzymes activities in citrus peel were also investigated. The results indicated that BTH treatment at three concentrations (0.25, 0.50, 1.00 mmol/L) could reduce lesion diameters of citrus fruits inoculated with three pathogens and the best effect was observed at 0.50 mmol/L. All these three treatments controlled disease incidence at the early stage of disease, but the effect was marginal at the late stage. By comparison, BTH at 0.50 mmol/L was most effective. BTH wound inoculation at the optimum concentration could increase the content of endogenous SA in the peel. BTH dipping treatment had no signifcant effect on the content of endogenous free SA in the peel, but could promote the accumulation of endogenous bound SA content. Both BTH treatments could increase the activities of β-1,3-glucanase (GLU), chitinase (CHI), phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in the peel. Therefore, BTH could be effective in inducing postharvest disease resistance in citrus fruits.
作者 刘晓佳 薛耀碧 周雅涵 姚世响 邓丽莉 曾凯芳 LIU Xiaojia1, XUE Yaobi1, ZHOU Yahan1, YAO Shixiang1, DENG Lili1,2, ZENG Kaifang1,2(1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Chongqing Engineering Research Center for Special Food, Chongqing 400715, Chin)
出处 《食品科学》 EI CAS CSCD 北大核心 2018年第9期215-223,共9页 Food Science
基金 "十二五"国家科技支撑计划项目(2015BAD16B07) 国家自然科学基金面上项目(31471631) 重庆市社会事业与民生保障科技创新专项(cstc2016shms-ztzx80005)
关键词 苯并噻二唑 柑橘果实 侵染性病害 诱导抗病性 benzothiadiazole citrus fruit infectious diseases induced resistance
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