阴性分数法和残存曲线法测定湿热灭菌用自含式生物指示剂D值的比较

Comparasion of Fraction Negative Method and Survivor Curve Method for the D Value Determination of Self-contained Biological Indicator for Steam Sterilization

分别采用阴性分数法和残存曲线法,测定湿热灭菌用自含式生物指示剂的D值,并对测定结果进行比较分析。阴性分数法和残存曲线法的测定结果分别为(2.22±0.10)min和(1.91±0.10)min,二者均高于标示值1.7 min。相比较而言,阴性分数法试验操作较为简便,但样品用量较大;而残存曲线法试验操作较为复杂,样品用量较小。通过分析比较可知,2种方法均适用于湿热灭菌用自含式生物指示剂的D值测定,可以根据实际情况和需求选择使用。

Fraction negative method and survivor curve method were used to determine the D value of self- contained biological indicator for steam sterilization. The results were compared and analyzed. The determined D values of fraction negative method and survivor curve method were （2.22±0.10） min and （1.91±0.10） min, both of which were higher than the labeled D value （1.7 min）. As a result, the operation for fraction negative method is simpler but it needs a larger amount of sample, meanwhile, the survivor curve method is more complicated to operate but its sample volume is smaller. Both of them can be used to determine D value of self-contained biological indicator for steam sterilization. Fraction negative method is recommended to be preferred, however, if the sample is insufficient, survivor curve method is an alternative method. Experimenters could choose the most suitable method according to the actual situation.