骨髓内皮祖细胞移植治疗糖尿病大鼠周围神经病变的机制探讨

The mechanism of bone marrow-derived endothelial progenitor cells in the treatment of peripheral neuropathy of diabetic rats

目的探讨骨髓内皮祖细胞（endothelial progenitor cells，EPCs）在糖尿病周围神经病变（diabetic peripheral neuropathy，DPN））治疗中的作用机制。方法用链脲佐菌素60mg／kg腹腔注射制备DPN大鼠模型。将雄性SD大鼠分为正常组（NC组）、DPN组、DPN＋EPCs组和DPN＋生理盐水组（DPN＋S组）。检测大鼠坐骨神经运动神经传导速度（motor nerve conduction velocity，MNCV）．以免疫组化检测NF．KB和髓鞘碱性蛋白（myelin basic protein，MBP）在坐骨神经的表达水平。结果与DPN组相比，DPN＋EPCs组的NF．KB表达明显减少（P〈0．05），DPN＋S组的NF—KB表达增加，差异有统计学意义（P〈0．01）。DPN＋EPCs组的MBP表达量与DPN组相比差异无统计学差异（P〉0．05），但较DPN＋S组增加（P〈0．01）。结论EPCs移植能抑制NF—KB、增加MBP的表达，这可能有利于神经损伤的修复。

Objective To explore the mecha-nism of bone marrow-derived endothelial progenitor cells （EPCs） in the treatment of peripheral neuropathy of diabetic rats. Methods Rats with diabetic peripheral neuropathy （DPN） were induced by streptozotocin （ 60 mg/kg）. Male SD rats were divided into normal control group （ NC group）, DPN group, DPN ＋ saline group （DPN ＋ S group）, and DPN ＋ EPCs group. Sciatic nerve motor nerve conduction velocity （MNCV） was measured. The expressions of NF-KB and myelin basic protein （MBP） in sciatic nerve were detected by immunohistochemistry. Results Compared with DPN group, the expression of NF-KB was reduced in the sciatic nerve of DPN＋ EPCs group, while the expression of NF-KB was increased in the sciatic nerve of DPN ＋ S group. There was no statistical difference in the expression of MBP between DPN and DPN ＋ EPCs groups. Compared with DPN ＋ S group, the expression of MBP was higher in DPN ＋ EPCs group. Conclusion Transplantation of EPCs inhibits the expression of NF-KB and increases the expression of MBP, which might be conducive to the repairs of nerve injury.