心肌肥厚大鼠FHL2的表达水平变化研究

The expression of FHL2 in rats with cardiac hypertrophy

目的研究大鼠心肌肥厚时FHL2表达水平的变化,探讨FHL2对心肌肥厚调控的作用。方法将雄性SD大鼠20只,随机分成腹主动脉缩窄组(AAC组)和假手术组(Sham组),每组10只,AAC组通过U型银夹缩窄腹主动脉的方法建立左室心肌肥厚模型,Sham组进行相同的手术步骤但未缩窄腹主动脉;术后6周,经胸心脏彩超测定心脏形态和心功能后以颈椎脱臼法处死,分离大鼠心脏记录形态学参数并通过HE染色和Masson染色观察心脏形态和纤维化程度;提取所有心肌组织总蛋白和总RNA,采用Western blot技术、实时定量PCR技术分析FHL2在两组大鼠心肌组织中的蛋白和mRNA表达水平。结果术后6周,心脏超声结果显示ACC组心肌较Sham组明显肥厚(P<0.05);与Sham组相比,AAC组大鼠心肌组织明显增厚,差异有统计学意义(P<0.05);ACC组FHL2蛋白和mRNA水平均明显较Sham组升高,差异有统计学意义(P<0.01)。结论心肌肥厚时FHL2表达水平明显上调;FHL2可能在心肌肥厚的发生发展过程中发挥重要调节作用。

Objective To study the expression of FHL2 rats with cardiac hypertrophy. Methods Twenty male Sprague -Dawley （SD） rats were randomly divided into the abdominal aortic constriction group （Group AAC, n = 10） and Sham group （Group Sham, n = 10）. The left ventricular hypertrophy model was established by implanting silver U clip on abdominal aorta in Group AAC, the same procedure except for silver U clip implanting was done in Group Sham. Transthoracic echocardiography was performed to detect the myocardial morphology and function 6 weeks after operation. The rats were sacrificed by cervical vertebra dislocation, and the hearts were harvested to measure the weight and analysis the relevant ratio. In addition, the myocardial tissues were detected in morphology with HE staining and Masson staining. The Western blot and real - time quantitative PCR analyze were used to detect the protein expression level and mRNA level of FHL2 in myocardial tissues. Results Compared with the Group Sham, there was a dynamic change of hypertrophy in Group ACC with a significant difference （P 〈 0.05）. The histological examination also showed significant increasing of cardiac hypertrophy in ACC group （P 〈 0.05 ）. Both the protein and mRNA levels in Group ACC were significantly higher than those in Group Sham. Conclusion The expression of FHL2 is significantly up - regulated in hypertrophic myocardi- um. FHL2 may play an important role in the development of cardiac hypertrophy.