脑功肽组方对高架十字迷宫大鼠海马单胺类递质及其代谢物的影响

Influence of Naogongtai Formula on Hippocampus Monoamine Neurotransmitters and Their Metabolites inRats in Elevated Plus-maze Test

目的:观察脑功肽组方对高架十字迷宫大鼠海马单胺类递质及其代谢产物含量的影响,探讨脑功肽组方抗焦虑作用的机制。方法:采用大鼠高架十字迷宫焦虑动物模型(EPM)观察脑功肽组方2个剂量组对大鼠焦虑行为学的影响;采用高效液相色谱-荧光检测(HPLC-FLD)法测定大鼠海马单胺类递质及其代谢产物的含量。结果:与空白对照组相比,脑功肽组方高剂量组进入开臂次数百分比、开臂停留时间百分比均显著提高(P<0.05),脑功肽低剂量组大鼠进入开臂次数百分比显著提高(P<0.05);脑功肽高剂量组大鼠海马5-羟色胺(5-HT)的含量与空白对照组相比显著降低(P<0.05),高香草酸(HVA)含量显著升高(P<0.05)。结论:在EPM实验中,脑功肽组方显示其具有一定的抗焦虑作用,大鼠海马单胺类递质含量测定显示可能与降低大鼠海马5-HT含量有关。

Objective： To observe the influence of Naogongtai formula on hippocampus monoamine neurotransmitters and their metabolites in rats in elevated plus-maze test, and explore the mechanism of antihypertensive effect of brain tegument peptide. Methods： The effects of two doses of Naogongtai formula on the anxiety behavior of rats were observed by using the rat model of elevated malaria anxiety （EPM）. The contents of monoamine neurotransmitters and their metabolites in hippocampus of rats were determined by HPLC-FLD. Results： Compared with those in the blank control group, the percents of open arms time and entries in the high dosage of Naogongtai formula group significantly increased （P 〈 0.05）, and the percent of open arms entries in the low dosage of Naogongtai formula group ＂significantly increased （P 〈 0.05）. Compared with that in the blank control group, the content of 5-HT in the high dosage of Naogongtai formula group significantly decreased （P 〈 0.05） and the content of HVA significantly increased （P 〈 0.05）. Conclusion： In the EPM test, Naogongtai formula shows a certain anti-anxiety effect, and the determination of monoamine neurotransmitters in hippocampus of rats may be related to the reduction of rat hippocampal 5-HT content.