肉桂酸与桂皮醛的平衡溶解度及表观油水分配系数的测定

Determination of Equilibrium Solubility and n-Octanol/Water Partition Coefficient of Cinnamon Acid and Cinnamaldehyde

目的:测定肉桂酸与桂皮醛的平衡溶解度及表观油水分配系数(P_(app))。方法:采用HPLC法测定肉桂酸与桂皮醛在不同pH的缓冲溶液中的平衡溶解度;结合摇瓶法测定其在不同pH缓冲盐溶液及正辛醇-水溶液中的表观油水分配系数。结果:在pH 7.8的缓冲溶液中肉桂酸的平衡溶解度最大,pH 6.8的缓冲溶液中桂皮醛的平衡溶解度最大;肉桂酸与桂皮醛在正辛醇饱和的水溶液中lg P_(app)值分别为0.85与1.26,在不同磷酸盐缓冲液中(pH 1.2~7.8)的lg P_(app)范围分别在-1.04~2.27,0.29~1.67之间。结论:此法简便、准确、快捷,可预测化学成分在体内的吸收;在胃肠道环境中,桂皮醛的吸收较好,肉桂酸在胃中的吸收较差,在肠道中吸收较好。

Objective： To determine the equilibrium solubility and n-octanol/water partition coefficients （Papp） of cinnamon acid and cinnamaldebyde. Methods： The equilibrium solubility of cinnamon acid and cinnamaldehyde in different solutions was determined by HPLC, and their n-octanol/water partition coefficients were determined by a shaking flask method combined with HPLC-DAD. Results： When the pH of solution was 7.8, the equilibrium solubility of cinnamon acid was the largest, while that of cinnamaldehyde was the largest in pH 6.8 solution. The scopes of lgPapp of cinnamon acid and cinnamaldehyde in different buffer solutions（pH 1.2- 7.8） were - 1.04- 2.27 and 0.29- 1.67, respectively, while those in n-octanol./water solvent were 0.85 and 1.26, respectively. Conclusion： The methoct is simple, accurate and fast to predicate the absorption of chemical components. In gastrointestinal physiological environment, cinnamaldehyde has good absorption, while cinnamon acid is with poor absorption in stomach and with better absorption in intestinal.