正交设计法优选童便炮制马钱子的工艺

Processing Technology Optimization for Strychni Semen with Children's Urine by Orthogonal Test

目的:优选童便炮制马钱子的工艺。方法:通过L_9(3~4)正交设计考察童便种类、童便用量、童便浸泡时间、烘干温度4个因素,以高效液相色谱法测定马钱子碱和士的宁含量作为考察指标,进行加权综合评分。采用方差分析对童便炮制马钱子工艺进行优选。结果:童便炮制马钱子的影响因素中浸泡时间的影响最明显,其余三因素对马钱子碱和士的宁含量无明显影响。最佳炮制工艺为A_1B_1C_3D_3。结论:童便炮制马钱子的最优工艺为选用3岁童便,3倍童便量,浸泡时间为10 d,烘干温度为90℃。本方法稳定,可靠,可用于马钱子的炮制新工艺。

Objective： To research the best processing method for Strychni Semen with children＇ s urine by orthogonal test. Methods： L9 （34） orthogonal test was used with the types of children＇s urine, dosage of children＇s urine, soaking time and drying temperature as the influencing factors, HPLC was applied to determine the contents of brucine and strychnine as the indices to obtain the comprehensive weighted score. ANOVA was used to optimize the processing technology of Strychni Semen with children＇ s urine. Results： The soaking time showed significant effect on the content of brucine and strychnine, while the other three factors had no significant effect. The best processing technology was A1B1C3D3. Conclusion： The optimum processing of Strychni Semen with children＇ s urine is as follows ： 3-year-old children＇ s urine with 3-fold amount is used to soak for 10 d, and the drying temperature is 90 ℃. The method is stable and reliable, which can be used for the new processing technology of Strychni Semen.