伤科止痛油提取工艺的优选

Optimization of the Extraction Process of Shangke Zhitong Oil

目的:优选伤科止痛油最佳提取工艺。方法:以毛蕊花糖苷和没食子酸提取量的综合评分为指标,采用L_9(3~4)正交试验考察乙醇浓度、浸泡时间、提取次数和提取时间对伤科止痛油的提取工艺的影响。结果:最佳提取工艺为用65%乙醇浸泡1 h后,再回流提取3次,第一次1.5 h,第二、三次1 h;毛蕊花糖苷和没食子酸提取量分别为0.870 8、0.717 8 mg·ml^(-1)。结论:优选所得的提取工艺简单易行,科学合理。

Objective： To optimize the extraction process of Shangke Zhitong oil. Methods： Taking the comprehensive score of extracting amounts of acteoside and garlic acid as the index, L9 （34） orthogonal test was used to observe the effects of alcohol concentration, soaking time, extraction times and extraction duration on the extraction results. Results： The best extraction process was as follows： the herbs were soaked for 1 h in 65% alcohol, and then refluxed and extracted for three times with 1.5 h for the first time, 1 hour for the second time and the third time. The extraction capacity of acteoside and garlic acid was 0. 870 8 and 0. 717 8 mg · ml-1 , respectively. Conclusion： The extraction process is simple, feasible, scientific and reasonable.