探讨P16/Ki67蛋白检测在宫颈上皮内瘤变诊断中的应用

Investigation on application of P16/Ki67 protein detection in the diagnosis of cervical intraepithelial neoplasia

目的探讨P16/Ki67蛋白检测在宫颈上皮内瘤变诊断中的应用价值。方法 16949例行宫颈液基细胞学检查者作为研究对象,全部细胞标本均行巴氏染色和P16及Ki67免疫细胞化学染色,以宫颈活检结果为金标准,观察P16及Ki67诊断结果。结果 16949例妇科细胞学检查者中全部细胞标本均行P16免疫化学染色,阴性14720例,阳性2012例,强阳性217例,总阳性率13.67%。在改良液基细胞系统基础上实施P16和Ki67蛋白检测,特异性明显。P16和Ki67蛋白检测的阳性表达率越高,CIN病变级别越高。结论在宫颈上皮内瘤变的诊断中,在改良液基细胞系统基础上实施P16和Ki67蛋白检测,具有重要的应用价值,可以明确CIN病变的分级,提高诊断正确率,降低漏诊率,为临床治疗提供科学的依据。

Objective To investigate the application value of P16/Ki67 protein detection in the diagnosis of cervical intraepithelial neoplasia. Methods A total of 16949 patients with thinprep cytologic test as study subjects. All cell specimens were stained with PAP staining, P16 and Ki67 immunocytochemistry. The cervical biopsy results were taken as the gold standard, and the diagnostic results of P16 and Ki67 were observed. Results In all 16949 cases of gynecological cytology, all the cell specimens were stained with P16 immunochemistry, with 14720 negative cases, 2012 positive cases, 217 strong positive cases, and the total positive rate was 13.67%. The detection of P16 and Ki67 proteins on the basis of a modified liquid-based cell system has obvious specificity. The higher the positive rate of P16 and Ki67 protein detection, the higher the CIN lesion level. Conclusion In the diagnosis of cervical intraepithelial neoplasia, detection of P16 and Ki67 protein in modified liquid based cell systems shows important application value, and it can identify the classification of CIN lesions, improve the accuracy of diagnosis, reduce the rate of missed diagnosis, and provide a scientific basis for clinical treatment.