CD64联合CD14检测在急性髓系白血病免疫分型鉴别中的应用价值

Application value of CD64 combined with CD14 in immunophenotyping diagnosis of acute myeloid leukemia

目的分析CD64联合CD14检测在急性髓系白血病免疫分型鉴别中的应用价值。方法选择急性髓系白血病患者82例为研究对象。应用流式细胞术对患者细胞CD14及CD64抗原表达情况进行检测,并分析免疫分型检测结果。结果 82例急性髓系白血病患者中,抗原表达CD64者白细胞计数高于CD14者,差异有统计学意义(P<0.05);CD14在M0型、M1型、M2型、M3型、M4型、M5型中的阳性表达率分别为0、0、0、0、56.52%、75.00%,CD64分别为0、0、15.38%、45.45%、82.61%、100%,CD64和CD14联合检测为0、0、15.38%、45.45%、86.96%和100%。结论 CD64联合CD14检测可提高M4型和M5型的鉴别诊断率,可指导临床制定治疗方案,在急性髓系白血病免疫分型鉴别中具有较高的应用价值。

Objective To analyze the application value of CD64 combined with CD14 in immu- nophenotyping diagnosis of acute myeloid leukemia. Methods Eighty-two patients with acute myeloid leukemia were selected as the study subjects. The antigen expressions of CD14 and CD64 of patients were detected by flow eytometry. Results In patients with acute myeloid leukemia, the white blood cell count of patients with antigen expressions of CD14 was higher than that of patients with antigen expressions of CD14, the difference was significant （ P 〈 0. 05 ） ; the positive expression rates of CD14 in M0, M1, M2, M3, M4 and M5 respectively were 0, 0, 0, 0, 56.52%, 75.00%, and those were 0, 0, 15.38% , 45.45% , 82. 61% and 100% of CD64. The combined detection of CD64 and CD14 were 0, 0, 15.38%, 45.45%, 86.96% and 100% respectively. Conclusions The detection of CD64 com- bined with CD14 has high value in the the differential diagnosis of acute myeloid leukemia and can im- prove the differential diagnosis rate between M4 and M5.