碳酸酐酶Ⅵ与4～5岁儿童龋病相关性的研究

Correlation between Carbonic AnhydraseⅥ Level and Dental Caries among Children Aged 4-5 Years

目的:初步探讨4~5岁儿童唾液、菌斑液中碳酸酐酶Ⅵ(CA-Ⅵ)含量与龋病的关系。方法:以无龋组、低龋组、高龋组儿童各33名为研究对象,采用ELISA法检测唾液、菌斑液中CA-Ⅵ含量,H+-ISFET测定菌斑液pH值,并进行统计学分析。结果:唾液CA-Ⅵ含量:无龋组>低龋组>高龋组,各组间有统计学差异(P<0.05);菌斑液CA-Ⅵ含量:无龋组>低龋组>高龋组,各组间有统计学差异(P<0.05);唾液、菌斑液CA-Ⅵ含量和菌斑液pH值呈线性正相关(P<0.001)。结论:随着龋病严重程度的增加,唾液、菌斑液CA-Ⅵ水平呈下降趋势。CA-Ⅵ可上调牙表面菌斑pH值,对保护牙齿、抵抗龋病有重要作用。

Objective：To investigate the relationship between dental caries and CA-Ⅵlevels in saliva and dental plaque fluid in children aged 4-5 years.Methods：Thirty-three children were randomly selected for each group：caries-free group,low caries group,and high caries group.The levels of CA-Ⅵin dental plaque and saliva were examined by ELISA.The pH values of dental plaque fluid were detected by H＋-ISFET.The results were statistically analyzed.Results：The CA-Ⅵ levels in saliva were statistically different among three groups：caries-free grouplow caries grouphigh caries group（P〈0.05）.The CA-Ⅵlevels in dental plaque fluid were significantly different among three groups：caries-free grouplow caries grouphigh caries group（P〈0.05）.Pearson＇s correlation analysis showed CA-Ⅵlevels in saliva and dental plaque fluid were positively related to pH values of dental plaque fluid（P〈0.001）.Conclusion：With the increase of caries severity,the levels of CA-Ⅵin saliva and dental plaque fluid decreased.CA-Ⅵ might increase the pH values of plaque on the dental surface,and play an important role in tooth protection and caries resistance.