小鼠牙乳头细胞向成牙本质细胞向分化过程中环状RNA的表达谱研究

Analysis of Circle RNA Expression Profile Variation during Odontoblastic Differentiation of Mouse Dental Papilla Cells

目的:探讨环状RNA(circle RNA,circRNA)在小鼠牙乳头细胞向成牙本质细胞向分化前后的表达差异,研究circRNA在成牙本质细胞发育过程中发挥的作用。方法:利用circRNA芯片技术检测矿化诱导组与未矿化组原代小鼠牙乳头细胞的circRNA表达谱,经过对原始数据进行预处理,均一化后,找出差异表达的circRNA。根据表达差异倍数较高和样本间一致性较好的原则筛选circRNA。结果:与未矿化组相比,矿化诱导组小鼠牙乳头细胞中2倍以上表达,并且差异具有统计学意义(P≤0.01)的circRNA定义为差异表达的circRNA。在经矿化诱导的牙乳头细胞中,差异表达circRNA共4064条,其中上调表达3255条,下调表达809条。结论:circRNA芯片可用于筛选牙乳头细胞向成牙本质细胞分化过程中差异表达的circRNA,在小鼠牙乳头细胞向成牙本质细胞向分化过程中,circRNA表达谱发生了显著变化,说明circRNA在成牙本质细胞发育过程中发挥着重要的调控作用。

Objective：To analyze the expression profile variation of circle RNA（circRNA）during odontoblastic differentiation of mouse dental papilla cells and further investigate the functions of circRNA during the process.Methods：CircRNA microarray was performed to detect the difference of circRNA expression between primary mouse dental papilla cells with odontoblastic differentiation inducing or not.After pretreatment and homogenization of raw data,the circRNAs with different expression were screened out and then analyzed.Results：Compared with noninducing dental papilla cells,there were 4064 circRNAs differentially expressed which were more than 2 times variation and significant difference（P≤0.01）in odontoblastic differentiation inducing cells.Among them,3255 were increased while 809 were reduced and the change′s fold was more than 2 times.Conclusion：The circRNA expression profile changed significantly during odontoblastic differentiation.The different circRNAs might be associated with the development of odontoblasts.