甲基丙烯酸羟乙酯对人牙髓组织的毒性及自噬研究

Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate

目的:探讨HEMA(甲基丙烯酸羟乙酯,2-Hydroxyethyl methacrylate)对牙髓的毒性作用及自噬相关机制。方法:构建新鲜健康年轻恒牙的切片器官培养模型,8 mmol/L HEMA刺激牙切片器官。CCK-8检测HEMA处理12、24、48h牙切片器官内牙髓组织的活力;苏木精-伊红染色检测48h后牙髓组织内剩余细胞密度;透射电镜观察不同时间段细胞亚显微结构的变化;组织荧光检测自噬标志分子LC3表达。结果:8 mmol/L HEMA明显抑制牙髓组织活性,并呈现时间依赖性,48h存活率仅为37.5%。HEMA处理后牙髓组织中细胞亚显微结构改变,并可见明显增加的自噬溶酶体。与对照组相比,HEMA处理组中LC3呈点状聚集,表达明显升高。结论:8mmol/L HEMA对人牙髓组织有明显的毒性作用,其机制与细胞自噬活化水平的升高有关。

Objective：To investigate the toxicity and possible mechanism of 2-hydroxyethyl methacrylate on human dental pulp in tooth slice organ culture model.Methods：Human tooth slice organ culture model was established in vitro,which was exposed to 8 mmol/L HEMA for 12 h,24 h,and 48 h.Separated dental pulp tissue viability was detected by CCK-8 assessment.Hematoxylin and eosin（HE）staining demonstrated the cell density change of dental pulp tissue from the ex vivo cultured tooth slice organ model.TEM analysis showed the ultrastructure change of dental mesenchymal cells during autophagy process.Immunostaining detected the LC3 expression in dental pulp.Result：8 mmol/L HEMA exposure decreased the viability and cell density of dental pulp tissue and increased autophagic vacuoles in a time-dependent manner.And the expression of autophagic biomarkers LC3 increased after 48 h.Conclusion：HEMA greatly decreased the tissue viability and induced autophagy in human dental pulp.