ApoC3基因高表达对小鼠急性胰腺炎严重程度的影响

Effects of ApoC3 on the severity of acute pancreatitis in mice

目的探讨ApoC3基因对高三酰甘油急性胰腺炎（AP）炎症严重程度的影响。方法采用雨蛙素腹腔注射法制备ApoC3转基因小鼠和C57BL／6J小鼠AP模型，等容积生理盐水腹腔注射ApoC3转基因小鼠和C57BL／6J小鼠作为对照组。检测各组小鼠血清三酰甘油和胆固醇水平，观察胰腺组织病理变化及采用RT—PCR法检测反映炎症损伤程度的炎症因子IL-1β、IL-6、α-SMA、TNF-α mRNA水平变化。结果ApoC3转基因小鼠的血清三酰甘油和胆固醇水平显著高于C57BL/6J小鼠[（3．434±0．931）mmol/L比（0．766±0．120）mmol/L，（2．553±0．178）mmol/L比（1．996±0．080）mmol/L]，差异均有统计学意义（P值均〈0．05）；ApoC3转基因小鼠AP组胰腺组织病理改变较C57BL／6J小鼠AP组严重；胰腺组织IL-1β、IL-6、α-SMA mRNA水平较C57BL／6J小鼠显著升高（1．72±0．07比0．78±0．09、1．58±0．09比0．87±0．04、0．83±0．05比0．44±0．04），差异均有统计学意义（P值均〈0．05），而TNF-α mRNA表达差异无统计学意义（0．70±0．09tL0．65±0．08，P〉0．05）。结论ApoC3基因可能加重高脂血症性胰腺炎的炎症程度。

Objective To explore the effects of ApoC3 gene on the severity of hypertriglycefidemia- induced acute pancreatitis （AP）. Methods ApoC3 transgenetic mice and C57BL/6J mice AP model was induced by cerulein intraperitoneal injection, and ApoC3 transgenetic mice and C57BL/6J mice injected by normal saline solution in equal volume served as control group. Serum triglyceride and cholesterol were detected, and the pathological changes of the pancreas were observed. RT PCR method was used to examine the changes of the inflammatory factor including IL-1β、IL-6、α-SMA and TNF-α mRNA levels, which reflected the severity of the inflammation. Results Serum triglyceride and cholesterol were higher in ApoC3 transgenetic mice than in C57BL/6J mice [ （ 3. 434 ± 0.931 ） mmol/L vs （ 0. 766± 0. 120 ） mmol/L, （ 2. 553± 0. 178 ） mmol/L vs （ 1. 996 ± 0. 080 ） mmol/L ] , and the differences were statistically different （ P 〈 0.05 ）. The pathological changes of the pancreas were more severe in ApoC3 transgenetic AP mice than in C57BL/6J AP mice, and the IL-1β、IL-6、α-SMA andTNF-α mRNA levels in the pancreatic tissue were obviously higher in ApoC3 transgenetic AP mice than in C57BL/6J mice （ 1.72 ±0.07vs 0. 78± 0.09, 1.58 ±0. 09vs 0. 87 ± 0.04, 0.83± 0.05vs 0. 44±0. 04） , and the differences were statistically significant （ P 〈 0.05 ） , while there was no statistical difference on TNF-cLmRNA level （ 0.70 ± 0.09vs 0. 65 ±0. 08, P 〉 0.05 ）. Conclusions ApoC3 gene could aggravate the severity of the inflammation in hypertriglyceridemia-induced AP.