抗抑郁剂对抑郁模型大鼠额叶炎性因子的影响

The effects of antidepressants on inflammatory factors of frontal lobe in depression model rats

目的探讨抗抑郁剂对抑郁模型大鼠额叶炎性因子的影响。方法将30只sD大鼠随机分为5组：正常对照组（Cn）、抑郁模型组（Dn）、西肽普兰组（Dx）、文拉法辛组（Dw）和瑞波西汀组（Dr），每组6只。用敞箱实验及糖水消耗实验进行行为学评价；用酶联免疫吸附实验检测各组大鼠额叶炎性因子浓度，用Pearson相关分析评估大鼠行为学评分与炎性因子的相关性。结果（1）与cn组比较，Dn、Dx、Dw、Dr组敞箱实验得分、糖水消耗量下降（P〈0．05）；与Dn组比较，Dx、Dw、Dr组敞箱实验得分、糖水消耗量升高（P〈0．05）；（2）与Cn组比较，Dn组成纤维细胞生长因子1（FGF-1）浓度降低，而肿瘤坏死因子α（TNF-α）、白介素1（IL-1）、C反应蛋白（CRP）浓度升高；与Dn组[（51．85±4．92）ng／L]相比，FGF-1浓度在Dx[（86．54±2．56）ng／L]、Dw[（79．82±4．89）ng／L]、Dr[（68．50±3．61）ng／L]组升高，而TNF-α浓度在Dx[（150．21±5．65）ng／L]、Dw[（161，28±8．80）ng／I．]、Dr[（175．78±9．67）ng／L]组降低，IL-1浓度在Dx[（30．87±4．48）ng／L]、Dw[（36．65±3．33）ng／L]、Dr[（40．14±2．81）ng／L]组降低，CRP浓度在Dx[（374．88±14.15）ng／L]、Dw[（394．21±17．03）ng／L]、Dr[（414．34±10．97）ng／L]组降低（P〈0．05）。（3）各组大鼠行为学评分与FGF-1浓度呈正相关，而与TNF-α、IL-1、CRP浓度呈负相关（P〈0．05）。结论抗抑郁剂可以降低抑郁模型大鼠额叶促炎因子水平，提高抗炎因子水平，提示抗抑郁剂可能通过调节炎性因子浓度来发挥抗抑郁作用。

Objective To investigate the effect of antidepressants on frontal lobe infflammatory factors in depressed model rats. Methods Thirty SD rats were randomly divided into 5 groups （n=6） ： contorl group（Cn） , depression group （ Dn ） , eitalopram group （ Dx ） , venlafaxine group （ Dw ） and reboxetine group （Dr） . The open field test and sugar consumption test were performed to observe the depression behavior and the changes of FGF-1 ,TNF-α,IL-1 and CRP were measured in frontal lobe of rats by ELISA. Pearson linear con＇elation analysis was used to evaluate the correlation between behavioral score and inflammatory factors level in rats. Results （ 1 ） Compared with the Cn group, the scores of Dn, Dr, Dw, Dx group were decreased on Open field test and sugar consumption test （P〈0.05）. Compared with the Dn group,the scores of the Dx, Dw, Dr group were increased in Open field test and sugar consumption test （P〈0.05）. （2）Compared with Cn group, the levels of TNF-α, IL-1 and CRP increased in Dn group and FGF-1 level decreased （P〈0.01）. Compared with Dn group,the levels of FGF-1 increased in Dx （（86.54±2.56） ng/L）,Dw（（79.82±4.89） ng/L） and Dr （（ 68. 50 ± 3. 61 ） ng/L ） group, however, the levels of TNF-α decreased in Dx （ （150.21±5.65） ng/L） ,Dw （ （161.28±8.80） ng/L） ,Dr （ （175.78±9.67） ng/L） group,and the level of IL- 1 also decreased in Dx （ （30.87±4.48） ng/L） , Dw （ （ 36.65±3.33 ） ng/L） , Dr （ （ 40.14±2.81 ） ng/L） group,and the levels of CRP also decreased in Dx （ （ 374.88 ±14.15 ） ng/L）, Dw （ （ 394.21 ± 17.03 ） ng/L）, Dr （ （414.34± 10.97）ng/L） （P〈0.01）. （3）The behavioral score of each group was positively correlated with of FGF-1 and negatively correlated with TNF-α,IL-1 and CRP （P〈0.05）. Conclusion Antidepressants can re- duce the level of proinflammatory factors and increase the level of anti-inflammatory factors of frontal lobe in depression model rats, suggesting that antidepressants may play an antidepressant effect by regulating the concentration of inflammatory factors.