lncRNA在抑郁症患者的差异表达与人格及社会支持的关系

Differential expression of lncRNAs in patients with depression and its relationship with personality traits and social support

目的探讨抑郁症患者lncRNA差异表达及其与人格特点、社会支持的关系。方法采用基因芯片筛查5例抑郁症患者（major depressive disorder，MDD）和5例正常对照（NC）之间有差异表达的lncRNA，根据标准选择其中10个lncRNAs，随后在连续入组的138例病人和43例正常人之间进行qRT—PCR验证，分析抑郁症患者lncRNA表达水平与人格特点、社会支持的相关性。结果基因芯片筛查到的2649个lncRNAs存在差异性表达，其中534个上调、2115个下调，在后续对10个lncRNAs的qPCR验证中，抑郁症中8个lncRNAs（TCONS_12_00001212（PY1）、NONHSAT102891（PY2）、TCONS_00019174（PY3）、ENST00000566208（PY4）、NONHSAG045500（PY6）、ENST00000517573（PY8）、NONHSAT034045（PY9）、NONHSAT142707（PY10））的表达水平存在显著下调（P〈0．05或P〈0．01）；PY4的ACt值与焦虑／躯体化因子呈负相关（r=-0．210，P〈0．05）；PY1、PY2、PY6的△Ct值与支持利用度呈负相关（r=-0．383，-0．391，-0．381，均P〈0．05）；除PY1外，其余各lncRNA的ACt值与偏执型呈负相关（P〈0．05）；PY3、PY6、PY9的ACt值与边缘型、强迫型呈负相关（P〈0．05）；除PY10外，其余各lncRNA的△Ct值与分裂型呈负相关（P〈0．05）。结论IncRNAPY1、PY2、PY3、PY4、PY6、PY8、PY9、PY10的表达水平在抑郁症患者呈明显下调，与焦虑／躯体化因子、人格特点和社会支持存在一定的相关性。

Objective To explore the differential expression of lncRNAs in patients with depression and its relationship with personality traits and social support. Methods The differential expression of lncRNAs in 5 patients with depression （MDD） and 5 normal controls （NC） was screened by gene chip.To validate the gene chip dataset,qRT-PCR was used to verify the expression levels of these 10 lncRNAs in a separate set of 138 consecutive patients and 43 normal subjects, and then the relationships of lncRNA expres- sion level with personality traits and social support were analyzed.Results A total of 2 649 lncRNAs were differentially expressed, of which 534 were up-regulated and 2 115 down-regulated.The expression levels of 8 lncRNAs analyzed by qRT-PCR in patients with depression were significantly down-regulated （P〈0.05 or P〈 0.01） .The △Ct value of PY4 was negatively correlated with anxiety/somatization factor （r=- 0. 210, P〈 0.05）, and the ACt values of PY1, PY2 and PY6 were negatively correlated with the social support availability （r=-0.383,-0.391 ,-0.381 all P〈0.05）.Apart from PY1 ,the ACt values of the other lncRNAs were negatively correlated with paranoid （P〈0.05）, the △Ct values of PY3, PY6 and PY9 were negatively correlated with the borderline and obsessive-compulsive （P〈0.05） and except for PY10,the △Ct values of the other In-cRNAs were negatively correlated with the schizoid （P〈0.05） .Conclusion The expression levels of theses 8 lncRNAs are significantly down-regulated in patients with depression, and there is a certain correlation with anxiety/somatization factor,personality traits and social support.