摘要
目的探讨抑郁症患者lncRNA差异表达及其与人格特点、社会支持的关系。方法采用基因芯片筛查5例抑郁症患者(major depressive disorder,MDD)和5例正常对照(NC)之间有差异表达的lncRNA,根据标准选择其中10个lncRNAs,随后在连续入组的138例病人和43例正常人之间进行qRT—PCR验证,分析抑郁症患者lncRNA表达水平与人格特点、社会支持的相关性。结果基因芯片筛查到的2649个lncRNAs存在差异性表达,其中534个上调、2115个下调,在后续对10个lncRNAs的qPCR验证中,抑郁症中8个lncRNAs(TCONS_12_00001212(PY1)、NONHSAT102891(PY2)、TCONS_00019174(PY3)、ENST00000566208(PY4)、NONHSAG045500(PY6)、ENST00000517573(PY8)、NONHSAT034045(PY9)、NONHSAT142707(PY10))的表达水平存在显著下调(P〈0.05或P〈0.01);PY4的ACt值与焦虑/躯体化因子呈负相关(r=-0.210,P〈0.05);PY1、PY2、PY6的△Ct值与支持利用度呈负相关(r=-0.383,-0.391,-0.381,均P〈0.05);除PY1外,其余各lncRNA的ACt值与偏执型呈负相关(P〈0.05);PY3、PY6、PY9的ACt值与边缘型、强迫型呈负相关(P〈0.05);除PY10外,其余各lncRNA的△Ct值与分裂型呈负相关(P〈0.05)。结论IncRNAPY1、PY2、PY3、PY4、PY6、PY8、PY9、PY10的表达水平在抑郁症患者呈明显下调,与焦虑/躯体化因子、人格特点和社会支持存在一定的相关性。
Objective To explore the differential expression of lncRNAs in patients with depression and its relationship with personality traits and social support. Methods The differential expression of lncRNAs in 5 patients with depression (MDD) and 5 normal controls (NC) was screened by gene chip.To validate the gene chip dataset,qRT-PCR was used to verify the expression levels of these 10 lncRNAs in a separate set of 138 consecutive patients and 43 normal subjects, and then the relationships of lncRNA expres- sion level with personality traits and social support were analyzed.Results A total of 2 649 lncRNAs were differentially expressed, of which 534 were up-regulated and 2 115 down-regulated.The expression levels of 8 lncRNAs analyzed by qRT-PCR in patients with depression were significantly down-regulated (P〈0.05 or P〈 0.01) .The △Ct value of PY4 was negatively correlated with anxiety/somatization factor (r=- 0. 210, P〈 0.05), and the ACt values of PY1, PY2 and PY6 were negatively correlated with the social support availability (r=-0.383,-0.391 ,-0.381 all P〈0.05).Apart from PY1 ,the ACt values of the other lncRNAs were negatively correlated with paranoid (P〈0.05), the △Ct values of PY3, PY6 and PY9 were negatively correlated with the borderline and obsessive-compulsive (P〈0.05) and except for PY10,the △Ct values of the other In-cRNAs were negatively correlated with the schizoid (P〈0.05) .Conclusion The expression levels of theses 8 lncRNAs are significantly down-regulated in patients with depression, and there is a certain correlation with anxiety/somatization factor,personality traits and social support.
作者
张琴
牛威
何明俊
孔令明
姚高峰
朱晓丽
张理义
Zhang Qin;Niu Wei;He Mingjun;Kong Lingming;Yao Gaofeng;Zhu Xiaoli;Zhang Liyi(Xuzhou Medical University, Xuzhou 221000, China ( Zhang Q;Department of Rehabilitation, No. 102 Hospital of Chinese People's Liberation Army, Changzhou 213003, China (Niu W;Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of the Chinese People's Liberation Army, Changzhou 213003, China (He MJ,Kong LM, Yao GF,Zhu XL,Zhang LY)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2018年第4期327-332,共6页
Chinese Journal of Behavioral Medicine and Brain Science
基金
全军后勤应用基础研究重大项目(BWS14J027)