枳葛解酒保肝方对酒精性肝损伤大鼠SOD、MDA、GSH的影响

Effects of Zhige Jiejiu Baogan Fang on SOD,MDA and GSH in rats with alcoholic liver injury

目的研究枳葛解酒保肝方对酒精性肝损伤大鼠肝脏的保护机制。方法选择雄性Wistar大鼠180只,随机分为空白组、模型组、阳性药组(0.36 g/kg)和枳葛解酒保肝方高、中、低剂量(18、9、1.8 g/kg)组。以浓度为50%的无水乙醇(以蒸馏水稀释)灌胃6周,建立大鼠酒精性肝损伤模型,以复方蛋氨酸胆碱片为阳性药,各给药组分别给予相应药物干预。检测血清及肝组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)含量,血清超氧化物歧化酶(SOD)活性。结果模型组大鼠血液中SOD的活性明显低于空白组,肝组织和血液中MDA、GSH含量明显高于空白组(P<0.01)。与模型组相比,枳葛解酒保肝方中剂量组能明显提高大鼠血清GSH含量(P<0.05)、提高SOD活力(P<0.01)和降低肝组织中MDA含量(P<0.01);枳葛解酒保肝方高剂量组能明显降低大鼠肝组织MDA含量(P<0.01)及提高GSH含量(P<0.01);枳葛解酒保肝方低剂量组能降低大鼠血清MDA含量(P<0.01)。结论枳葛解酒保肝方具有一定的增强SOD、GSH等内源性抗氧化酶的抗氧化能力,抑制自由基介导的脂质过氧化反应对肝脏损伤的作用。

Objective To investigate the protective effects of Zhige Jiejiu Baogan Fang（ Semen Hoveniae and Radix Puerariae Lobatae hangover-relieving and liver-protecting recipe,ZGJJBGF） on superoxide dismutase（ SOD）,malondialdehyde（ MDA） and glutathione（ GSH） rats with alcoholic liver injury.Methods 180 male Wistar rats were randomly divided into 6 groups： blank group,model group,positive control（ Compound Methionine and Choline Bitartrate Tablet） group（ 0. 36 g/kg）,high-dose,mid-dose and low-dose ZGJJBGF groups（ 18,9,1. 8 g/kg respectively）. Except for the blank group,rats in other groups were orally administered with 50% ethanol（ diluted with distilled water） for 6 weeks to establish the alcoholic liver injury rat model. Rats in all experimental groups except the blank and model groups received medicinal intervention respectively. The activity of malondialdehyde（ MDA）,glutathione（ GSH）,and superoxide dismutase（ SOD） in serum and liver were measured at the end of the experiment. Results SOD activity in model group was significantly lower than that in the blank group,and the contents of MDA and GSH in the liver tissues and blood were significantly higher in the blank group（ P〈0. 01）. Compared with model group,mid-dose ZGJJBGF significantly increased the serumGSH level（ P〈0. 05） and the activity of SOD（ P〈0. 01） while reducing the content of MDA in liver tissue（ P〈0. 01）. Compared with the model group,high-dose ZGJJBGF significantly reduced the content of MDA in liver tissue of rats（ P〈0. 01） and increased the content of GSH（ P〈0. 01）. Compared with model group,low-dose ZGJJBGF reduced serum MDA content（ P〈0. 01）. Conclusion Zhige Jiejiu Baogan Fang could enhance the antioxidant ability of endogenous antioxidant enzymes such as SOD and GSH. It can also inhibit the damage of liver injury of free radical-induced lipid peroxidation.