杨树PtRRI基因的组织特异性表达模式分析

Analysis of Tissue-specific Expression of Poplar PtRRI Gene

[目的]模式植物在木本植物中鉴定的许多重要调控因子家族在木本植物中出现了基因家族成员扩张,但ARRs家族作为细胞分裂素响应调节因子在杨树基因组中成员数量反而减少,其在木本植物中如何行使功能需要进一步研究。[方法]本研究通过生物信息学构建PtRRI启动子与GUS融合表达载体,检测植物激素处理后PtRRI表达量和检测PPtRRI::GUS转基因植株在生根过程中GUS信号等方法,对杨树PtRRI基因的组织特异性表达模式进行分析。[结果]表明:PtRRI在杨树根部、形成层、木质部表达量相对较高,PtRRI转录受6-BA激素诱导,与其在不定根发育过程中激素调控下的表达相一致。[结论]PtRRI基因可能参与杨树的次级生长。

[ Objective ] Many genes coding for important regulatory factors in model plant appears amplified numbers in woody plants. It is worth to be further investigated that the Class A ARR gene family, as a kind of cytokinin re- sponsive factor, reduces its number in Populus. [ Method ] Based on the method of bioinformaties, the expression pattern of family members was analyzed by RNA-seq and semi-quantitative PCR, building PtRRI fused to GUS ex- pression vector, Detecting the PtRRI expression in Populus alba x P. glandulosa clone after treated with 6-BA, GA3 , ABA and IBA hormones and the GUS signals of PPtRRI ： GUS transgenic plant in the process of rooting. [ Re- suit] The GUS signal in PPtRRI ： GUS transgenic plants indicated that the PtRRI was mainly expressed in roots, cambium, xylem and this gene may involve in the secondary growth and the expression pattern during adventitious root formation. In addition, the PtRRI transcription expression was induced by 6-BA, which was consistent with its expression under hormone regulation during adventitious root development. [ Conclusion] The results of this study suggest that PtRRs may involve in the process of secondary growth and development of Populus.