利用表面预烯基化纳米硅胶颗粒制备有机与无机杂化强阳离子交换液相色谱整体柱

Preparation of Organically-inorganically Hybrid Strong Cation-exchange Monolithic Column for Liquid Chromatography Using Pre-alkenylated Silica Nanoparticles

利用3-磺酸丙基甲基丙烯酸钾盐(SPMA)为有机功能单体,表面预烯基化的无机纳米硅胶颗粒(直径25~50nm)为交联剂,偶氮二异丁腈(AIBN)为引发剂,环己醇、甲醇和水为三元致孔剂,在内径为150μm石英毛细管内通过原位聚合的方法制备了一种有机与无机杂化强阳离子交换整体柱.最佳制备条件是:聚合混合物组成,纳米硅胶颗粒(14.4mg)+SPMA(3.6mg)+环己醇(22.56μL)+甲醇(7.52μL)+水(1.92μL)+AIBN(0.125mg);反应时间,7h;反应温度,60℃.通过分离一组无机阳离子和4种核苷酸对制得的整体柱色谱性能进行了评价.

An organic and inorganic hybrid strong cation exchange monolithic column was prepared for liquid chromatography by in situ polymerization of 3 sulfopropyl methacrylate potassium salt（SPMA） and pre alkenylated silica nanoparticles （i. d. 25 50 nm） in the presence of ternary porogens consisting of cyclohexanol , methanol and water in a 150μm i. d. fused silica capillary. The optimal preparation condi tions of the column were： composition of polymerized mixtures, inorganic silica nanoparticles（14.4 mg）, SPMA （3. 6 mg）, cyclohexanol （22.5G μL）, methanol （7.52 μL）, water （1.92 μL） and 2,2 Azobisisobu tyronitrile （0. 125 mg） ; polymerization time, 7 h ; polymerization temperature, 60℃. The separation per formance of the column was evaluated through the separation of monovalent inorganic cations and four nu cleotides.