摘要
鱼类的染色体核型分析是鱼类遗传学研究的基础,作为我国重要养殖品种的双斑东方鲀(Takifugu bimaculatus)尚未发现有染色体核型的相关报道.采用体内注射植物血球凝集素(PHA)及秋水仙素法制备了双斑东方鲀头肾组织染色体标本并分析其核型.结果表明,双斑东方鲀二倍体染色体数为2n=44,其染色体臂数为NF=62,核型公式为12m+6sm+26t.通过比较分析推测2n=44应该是东方鲀属的染色体基础核型,而其染色体核型的高度保守可能是东方鲀属鱼类易于发生种间杂交的主要原因之一.
Karyotype is the foundation of fish genetics. As one of the important species for aquaculture,the karyotype study of Takifugu bimaculatus has little been reported. In this study,the metaphase chromosomes of T. bimaculatus was obtained from head kidney tissue by the method of phytohemagglutinin（PHA） and colchicine injection,and then the karyotype was analyzed. The results show that edthatthere was of 44 chromosomes in the diploid and the karyotype formula was 12 m ＋ 6 sm ＋ 26 t,NF = 62. With results of comparison and analysis,it is suggested that2 n = 44 is the chromosome karyotype of Takifugu. The highly conservative of chromosome karyotype may be the one main reason of easily interspecific hybridization in genus Takifugu.
出处
《应用海洋学学报》
CSCD
北大核心
2018年第2期274-278,共5页
Journal of Applied Oceanography
基金
厦门南方海洋研究中心资助项目(13GYY002NF07)
海洋经济创新发展区域示范专项资助项目(12PYY001SF08-XMDX-2)
关键词
海洋生物学
双斑东方鲀
染色体
核型
遗传
杂交
marine biology
Takifugu bimaculatus
chromosome
karyotype
genetics
hybridization
