再生丝素蛋白溶液脱盐新工艺及其应用

Novel desalination process and application of regenerated silk fibroin solution

为提高丝素蛋白溶液的脱盐效率,降低脱盐能耗,采用扩散渗析-电渗析集成膜分离技术对丝素蛋白溶液进行脱盐,并初步探索所得溶液的应用。研究了丝素蛋白溶液质量分数和扩散渗析预脱盐率对脱盐效率和能耗的影响,同时将所得丝素蛋白溶液加入无水乙醇制备不溶性丝素蛋白粉,测定了产品氨基酸的组成并利用扫描电子显微镜对丝素蛋白粉进行表征。结果表明:最佳的脱盐技术参数为丝素蛋白溶液质量分数为5%、扩散渗析预脱盐率为40%,在此工艺条件下脱盐时间缩短至9 h,能耗(动力能耗除外)为3.81×10^(-2)kW·h/L;产品中丝氨酸和甘氨酸含量接近50%,丝素蛋白粉末粒径较小且分布均匀。

In order to improve the efficiency and reduce the energy consumption in the desalination of fibroin solution,the diffusion dialysis and electrodialysis membrane separation technique were applied to the desalination process,and the application properties of the silk fibroin solution were preliminarily explored.Factors affecting the desalting efficiency and energy,such as the concentration of silk fibroin and the pre-desalination rate were investigated.Moreover the insoluble silk fibroin powder was prepared by adding anhydrous ethanol to the silk fibroin solution,the amino acid composition of the product was determined and the silk fibroin powder was characterized by scanning electron microscopy.The results show that the optimal parameters for desalting is 5% of the concentration of silk fibroin and 40% of the pre-desalination rate.Under this condition,the desalination time is shortened to 9 h and the energy consumption（except dynamic power consumption） is 3.81 × 10^-2 kW·h/L; the total content of serine and glycine in the product is close to 50%,and scanning electron microscopic results show that the particle size of the powder is smaller and the distribution is uniform.