两种药剂亚致死浓度对稻纵卷叶螟生长发育及解毒酶活性的影响

Effects of Sublethal Concentration of Two Insecticides on Growth and Development of Rice Leaffolders, Cnaohalocrocis medinalis(Guenée) and Activities of Detoxification Enzymes

【目的】为了明确毒死蜱和氯虫苯甲酰胺对稻纵卷叶螟的亚致死效应,【方法】我们采用离体叶片浸渍法,2龄稻纵卷叶螟幼虫在亚致死浓度(LC_(10)和LC_(25))浸渍下的水稻叶片上处理72 h,然后观察稻纵卷叶螟的生长发育状况,并测定了其体内主要解毒酶活性。【结果】毒死蜱亚致死浓度LC_(10)和LC_(25)处理组幼虫历期较对照显著延长;蛹历期显著短于对照,雌雄成虫寿命与对照无显著差异。氯虫苯甲酰胺亚致死浓度LC_(10)和LC_(25)处理组,幼虫历期和蛹历期与对照均无显著差异;LC_(10)处理组雌成虫寿命缩短。毒死蜱LC_(10)和LC_(25)处理组,稻纵卷叶螟体内的酯酶和谷胱甘肽转移酶活性均显著高于对照。氯虫苯甲酰胺LC_(10)和LC_(25)处理后其体内的酯酶活性显著增加;LC_(25)处理组的谷胱甘肽转移酶活性显著高于对照。【结论】研究结果表明,毒死蜱和氯虫苯甲酰胺亚致死浓度处理使稻纵卷叶螟部分生长期发育放缓,但诱导体内部分解毒酶活性上升。稻纵卷叶螟处于这两种杀虫剂亚致死浓度的选择压下,将有利于抗药性的积累与发展。

【Objective】In order to understand the sublethal effect of chlorpyrifos and chlorantraniliprole on rice leaffolders Cnaphalocrocis medinalis, 【Method】the development was observed and detoxification enzymatic activities were measured observed after treatments with sublethal concentration of insecticides （LC10 and LC25） for 72 h. 【Results】In the treatments with LC10 and LC25 of chlorpyrifos, the larval duration prolonged and pupal duration shortened, the adult longevity did not differ compared with the control. LC10 and LC25 of chlorantraniliprole did not affect the larval and pupal duration, while LC10 of chlorantraniliprole shortened the longevity of female adult. Esterase and glutathione S-transferase （GST） activity of C. medinalis treated with LC10 and LC25 of chlorpyrifos increased compared with the control. Esterase activity of C. medinalis treated with LC10 and LC25 of chlorantraniliprole increased compared with the control;GST activity of C. medinalis treated with LC25 of chlorantraniliprole increased compared with the control. 【Conclusion】These results indicated that sublethal concentration of chlorpyrifos and chlorantraniliprole could inhibit the development of partial stages of C. medinalis, and induce the increase of detoxified enzymatic activities. Selective pressure of sublethal concentrations of these two insecticides on C. medinalis could promote the evolution of insect resistance against insecticides.