姜黄素处理对七氟醚引起的成年小鼠记忆提取障碍的影响

Pre-administration of Curcumin Improves Sevoflurane-induced Memory Retrevail Dysfunction of Adult Mice

目的:观察姜黄素对七氟醚引起的成年小鼠记忆提取障碍的影响。方法:成年昆明小鼠120只,雌雄各半,随机分为对照组(Con组),七氟醚组(Sev组)和七氟醚加姜黄素组(Cur组);Cur组连续6 d腹腔注射0.3 mg/g的姜黄素,Con组和Sev组注射等体积的二甲基亚砜和生理盐水混合液,之后Sev组、Cur组吸入3.3%七氟醚2 h,Con组吸入40%O_22 h;3组小鼠于吸入前及停止吸入后12、24、48及72 h进行避暗实验,记录各组小鼠5 min内错误次数、步入潜伏期及Δ潜伏期,评价小鼠记忆提取能力。结果:与Con组比较,Sev组吸入七氟醚后12、24 h步入潜伏期缩短,Δ潜伏期负值增大和5 min内错误次数增加(P<0.05);与Sev组比较,Cur组吸入七氟醚后12、24 h步入潜伏期延长,Δ潜伏期负值减小和5 min内错误次数减少(P<0.05);而吸入七氟醚后48、72 h时,Sev组、Cur组步入潜伏期和错误次数比较,差异无统计学意义(P>0.05)。结论:姜黄素预处理可改善七氟醚引起的成年小鼠短期记忆提取障碍。

Objective： To observe the effect of curcumin on sevoflurane-induced memory retrieval dysfunction in adult mice. Methods： Adult Kun Ming mice of 120 with both sexes were randomly divided into three groups： control group（ group Con）,sevoflurane group（ group Sev） and sevoflurane ＋curcumin group（ group Cur）. According to the different time of the test,mice in group Cur were made an intraperitoneal injection of curcumin with the dose of 0. 3 mg/g for 6 consecutive days while group Con and Sev were injected into isometric mixed liquids of dimethyl sulfoxide and mormal saline,then group Sev and group Cur were exposed to 3. 3% sevoflurane for 2 h and group Con inhaled 40% O2 for 2 h. The three groups were made step-through test before inhalation and after inhalation at 12 h,24 h,48 h,72 h respectively. Stepping-in the lantency,Δlantency and error times in 5 minutes of all groups were tested to evaluate the capability of memory retrieval in mice. Results： Compared with group Con,group Sev at 12 h and 24 h after inhalation with sevoflurane showed significant differences（ P〈0. 05）,stepping-in latency was decreased,Δlantency and error times in 5 minutes were increased; group Cur at 12 h and 24 h after inhalation with sevoflurane also showed significant differences（ P〈0. 05）,stepping-in latency was prolonged and Δlantency was increased compared with group Sev. But there was no significance in statistics comparing the stepping-in lantency with error times of Group Sev and Cur after inhalation with sevoflurane at 48 h and 72 h（ P〉0. 05）. Conclusion： Pre-administration of Curcumin can improve sevoflurane-induced memory retrevail dysfunction of adult mice.