接头蛋白Crk Ⅰ在喉鳞癌中的表达及意义

Expression and Significance of Crk Ⅰ in Laryngeal Squamous Cell Carcinoma

目的:探讨接头蛋白Crk Ⅰ在喉鳞癌组织中的表达及意义;方法:采用免疫组化SP法,对54例喉鳞癌手术切除标本和22例癌旁正常组织进行检测,观察Crk Ⅰ在2种组织中的表达,分析Crk Ⅰ的表达与鳞癌临床分期、病理分级、肿瘤转移及生存时间的关系。结果:Crk Ⅰ在喉癌组织中主要表达于细胞质、细胞核可见少量表达、阳性率达64.8%,在癌旁正常组织中少量表达或无表达,2种组织中Crk Ⅰ表达阳性率比较,差异有统计学意义(χ~2=24.149,P<0.01);喉鳞癌组织中,有淋巴结转移者Crk Ⅰ蛋白阳性表达率为91.7%,与无淋巴结转移者的54.8%比较,差异有统计学意义(P<0.05);但Crk Ⅰ蛋白的阳性表达率在各年龄组、不同临床分期、病理分级及不同预后的喉鳞癌患者中比较差异无统计学意义(P>0.05)。结论:接头蛋白Crk Ⅰ可能在喉鳞癌细胞的发生、发展和转移中有重要意义。

Objective： To investigate the expression and significance of Crk Ⅰ in laryngeal squamous cell carcinoma. Methods： Fifty-four cases with laryngeal cancer were detected by immunohistochemistry S-P method and the ablated laryngeal squamous cell carcinoma samples and the normal para-carcinoma tissues were analyzed. Results： Analysis showed that the expression of Crk Ⅰgene in laryngeal squamous cell carcinoma was up-regulated. The difference of Crk Ⅰin the tissues and laryngeal carcinoma of the control group was statistically significant. There was significant difference in lymph node metastasis. Conclusion： Over-expression of Crk Ⅰmay play an important role in the oncogenesis,progression and metastasis of laryngeal carcinoma.