新辅助化疗对宫颈癌患者外周血Treg细胞、血清TGF-β1、VEGF水平及组织VEGF、CD105表达的影响

Effect of Neoadjuvant Chemotherapy on Peripheral Blood Treg Cells, Serum Levels of TGF-[I1 and VEGF, and Expression of VEGF and CD105 in Cervical Cancer Tissues

【目的】探讨新辅助化疗对宫颈癌患者外周血Treg细胞、血清TGF-β1、VEGF水平及组织VEGF、CD105表达的影响。【方法】选取2014年3月至2017年3月期间在本院进行手术治疗的97例宫颈癌（Uterine Cervical Cancer,UCC）患者为研究对象,所有患者均采用新辅助化疗;以70例健康人为健康对照组（Healthy control,HC）。流式细胞仪检测三组外周血Treg/CD4＋细胞比例,Elisa法检测血清TGF-β1、VEGF水平,免疫组化法检测癌组织VEGF、CD105表达。【结果】UCC组新辅助化疗前外周血Treg/CD4＋细胞比例、TGF-β1、VEGF水平高于HC组,其差异具有统计学意义（P〈0.05）。新辅助化疗后UCC组患者外周血Treg细胞比例、血清TGF-β1、VEGF水平均较化疗前降低,差异具有统计学意义（P〈0.05）。化疗后UCC组患者癌组织标本中VEGF及CD105表达水平较化疗前明显降低,差异具有统计学意义（P〈0.05）。Pearson相关性分析表明,VEGF及CD105在UCC中的表达呈正相关关系（r=0.557,P=0.032）。【结论】新辅助化疗可降低UCC患者外周血Treg/CD4＋细胞比例,减少TGF-β1的释放,进而降低肿瘤病灶中CD105及VEGF的表达,抑制肿瘤血管生成,有利于缩小肿瘤病灶。

【Objective】To study the influence of neoadjuvant chemotherapy on the ratio of Treg / CD4 cells in the peripheral blood, serum levels of TGF-β1 and VEGF, and the expression of VEGF and CD105 in tumor tissues.【Methods】A total of 97 patients with uterine cervical cancer （UCC） who were treated in our department from Mar 2014 to Mar 2017 were enrolled in this study （UCC group）; all patients received neoadjuvant therapy. Meanwhile, 70 healthy women were included in the control group （HC group）. The ratio of Treg/ CD4＋ cells was measured by the flow cytometry assay, ; serum levels of TGF-β1 and VEGF were detected by ELISA assay, and the expression of VEGF and CD105 in the tumor tissue was evaluated by the immunohistochemistry.【Results】Before the neoadjuvant therapy, the ratio of the peripheral Treg/CD4 cells and the serum levels of TGF-β1 and VEGF were significantly higher in the UCC group than in the HC group （P〈0.05）. The ratio of Treg/CD4 cells and the serum levels of TGF-β1 and VEGF were significantly decreased after the neoadjuvant therapy （P〈0.05）. The expression of VEGF and CD105 in the tumor tissue of the UCC group significantly decreased after neoadjuvant therapy （P〈0.05）. A positive correlation between VEGF and CD105 expression in the UCC tissue was found by the Pearson correlation analysis （r=0.557, P=0.032）.【Conclusion】Neoadjuvant therapy can decrease the ratio of the peripheral Treg/CD4＋ cells, reduce the release of TGF-β1, and downregulate the expression of CD105 and VEGF in the UCC tissue, thereby inhibiting angiogenesis and improving remission.