猪红细胞免疫黏附功能与CR1-like表达水平的研究

Study on immune adhesion function and CR1-like expression level of porcine erythrocytes

本研究旨在探讨不同长白猪红细胞CR1-like表达量的差异性,以及CR1-like表达量与免疫黏附功能的关系,为后期研究猪红细胞CR1-like的免疫机理提供理论数据。运用流式细胞术检测长白猪红细胞免疫黏附荧光大肠杆菌的水平及红细胞CR1-like表达量,运用单因素方差分析、单样本Kolmogorov-Smirnov检验等多种统计方法分析红细胞CR1-like表达量的特点及其与免疫黏附功能之间的相关性。结果,猪红细胞免疫黏附功能在个体水平上分为低,中和高三种类型,各种类型的平均荧光值依次为33.897±3.294（34.483%）、68.076±12.145（46.552%）和129.232±8.143（18.966%）;CR1-like表达量在个体水平上分为低,中和高三种类型,各种类型的平均荧光值依次为21.479±0.865（14.583%）、27.984±2.218（81.034%）和36.690±0.058（6.897%）;相关性分析结果显示,红细胞免疫黏附水平与CR1-like表达水平达到显著相关程度（r=0.835,P〈0.01）,通过回归分析,获得二者符合典型的S曲线（lny=7.817-102.911x,r2=0.729,P〈0.01）。上述结果表明,不同的长白猪个体的红细胞CR1-like表达量具有差异性,且该特点与猪红细胞免疫黏附水平具有相关性。

To provide theoretical data for subsequent research on the molecular mechanisms underlying the immune function of porcine erythrocyte CR1-like,in this study,we investigated the variability of porcine erythrocyte CR1-like at the individual level and determined its relationship with the immune adhesion of porcine erythrocytes.Flow cytometry was used to analyze the immune adhesion levels between Landrace porcine erythrocytes and fluorescent Escherichia coli and to determine the expression levels of erythrocyte CR1-like.One-way ANOVA,one-sample Kolmogorov-Smirnov test,and other statistical methods were used to compare the expression characteristics of erythrocyte CR1-like and its correlation with immune adhesion functions.Porcine erythrocyte immune adhesion could be classified into three types at the individual level：low,moderate,and high,with mean fluorescence values of 33.897±3.294（34.483%）,68.076 ±12.145（46.552%）,and 129.232 ±8.143（18.966%）,respectively.CR1-like expression levels were also classified into three types at the individual level：low,moderate,and high,with mean fluorescence values of 21.479±0.865（14.583%）,27.984±2.218（81.034%）,and 36.690±0.058（6.897%）,respectively.There was a significant correlation between erythrocyte adhesion levels and CR1-like expression levels（r=0.835,P0.01）,and regression analysis showed that both variables obey a classical S-shaped curve（lny =7. 817-102. 911 x,r2=0. 729,P 0. 01）.In conclusion,variability in expression levels of erythrocyte CR1-like among Landrace porcine individuals are variable,which is correlated to porcine erythrocyte immune adhesion levels.