摘要
通过化学交联-溶液流延法,以环氧氯丙烷为交联剂与壳聚糖季铵盐(HTCC)反应,成功制备了交联壳聚糖季铵盐(CHTCC)抗菌薄膜.利用全反射红外光谱(ATR-FTIR)、核磁共振氢谱(1 H NMR)、扫描电镜(SEM)、平衡溶胀法、平板计数法和微量肉汤稀释法分析了CHTCC薄膜的结构、形貌、水中的稳定性和抗菌性.结果表明,交联剂用量与薄膜的交联度成正比.与HTCC薄膜相比,CHTCC薄膜在水中只发生溶胀而不溶解,对大肠杆菌(E.coli)、金黄色葡萄球菌(S.aureus)和铜绿假单胞菌(P.aeruginosa)都具有优异的抗菌活性,其最小抑菌浓度(MIC)分别为320,2 560和160μg/mL.制备的CHTCC可作为抗菌涂层、过滤膜以及药物缓释载体分别应用于食品包装、水处理和制药等领域.
An antibacterial cross-linked chitosan quaternary ammonium salt was prepared using epichlorohydrin as cross-linking agent and reacting with chitosan quaternary ammonium salt by chemical crosslinking and solution casting method.ATR-FTIR,^1 H NMR,SEM,equilibrium swelling method,plate count method and broth microdilution method were applied to analysis the structure,morphology,water stability and antimicrobial activity of cross-linked chitosan quaternary ammonium salt film.The results show that the amount of cross-linking agent is proportional to the cross-linking degree of the film.Compare with the chitosan quaternary ammonium salt film,cross-linked chitosan quaternary ammonium salt films only swell and not dissolve in water.And it maintains excellent antibacterial activity against E.coli,S.aureus and P.and aeruginosa,the minimum inhibitory concentrations are 320,2 560 and160μg/mL,respectively.Prepared cross-linked chitosan quaternary ammonium salt can be used as antibacterial coating,filter membrane and drug delivery carrier,which can be applied in food packaging,water treatment,pharmaceutical and other fields.
作者
解冰
夏凯
赵春毛
李洁
XIE Bing;XIA Kai;ZHAO Chun mao;LI Jie(School of Materials Science and Engineering, North University of China, Taiyuan 030051, China)
出处
《中北大学学报(自然科学版)》
CAS
2018年第2期230-235,240,共7页
Journal of North University of China(Natural Science Edition)
基金
山西省国际科技合作资助项目(201703D421007)
中北大学科学研究基金资助项目(2017010)