微波法提取木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的工艺研究

Study on Microwave Extraction Technology of Gypsogenin-3-O-Glucuronide from Momordica Cochinchinensis

目的:建立木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的微波提取方法,为木鳖子药材的进一步开发利用提供实验依据。方法:实验采用微波提取技术,以丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯含量为指标,考察乙醇浓度、微波功率、提取时间及提取温度对提取工艺的影响。结果:丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的线性范围为0.024~0.120 mg,平均加样回收率为100.2%。微波提取的最佳提取工艺为35%乙醇65℃,400 w提取12 min,平均提取率为0.267%。结论:微波法提取木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的方法可靠稳定,优选出的提取工艺简单、节能、省时可行。

Objective： To establish the microwave extraction method of gypsogenin-3-O-glucuronide from momordica cochinchinensis, and provide the experimental basis for the further development of momordica cochinchinensis. Methods： Using microwave extraction technology, we studied the effects of ethanol concentration,microwave power, extraction time, extraction temperature on extraction process of gypsogenin-3-O-glucuronide.Results： Linear range of gypsogenin-3-O-glucuronide was 0.024 ~0.120 mg, and the average recovery rate was 100.2%. The best extraction process was to extract 12 minutes with 35% ethanol, 65 ℃ and 400 w. The average extraction rate was 83.4%. Conclusion： The microwave extraction method was reliable and stable to extract the gypsogenin-3-O-glucuronide from momordica cochinchinensis. At the same time, this optimized extraction process was simple, energy conversation, time-saving and feasible.