不同龄大鼠骨髓间充质干细胞活性对比

The Comparison of Bone Marrow Mesenchymal Stem Cell in Ossification Activity

目的:分别在低氧环境和正常氧环境下研究不同周龄SD大鼠骨髓间充质干细胞(BMSCs)的细胞活性以及细胞分化能力的差别,并观察和检测不同周龄大鼠的骨髓间充质干细胞成骨活性有何差异。方法:1)、进行不同周龄SD大鼠骨髓间充质干细胞的提取及在低氧环境及正常氧环境下的培养。2)、进行细胞生物活性的测定及比较。结果:低氧环境下培养的2周4周龄大鼠的骨髓间充质干细胞(BMSCs)活性好于常氧状态下培养的2周4周龄大鼠的骨髓间充质干细胞(BMSCs)。结论:低氧环境下培养的年轻大鼠的骨髓间充质干细胞活性较好。

Objective： Comparative study of different week-old sd rat bone marrow mesenchymal stem cells （MSCs） cell activity and cell differentiation respectively in hypoxia environment and normal oxygen environment. Methods： 1,Extract different week-old SD rat bone marrow mesenchymal stem ceils and culture the cells respectively in hypoxia environment and normal oxygen environment. 2, Points out the best age of SD rat bone marrow mesenchymal stem cells biological activity. Results： The biological activity and osteogenic cells differentiation of 2 weeks and 4 weeks of sd rat bone marrow mesenchymal stem cells are better than those of 8 weeks, 12 weeks and 40 weeks especially in hypoxia environment. Conclusion： The experiment proves that biological activity of 2 weeks and 4 weeks sd rat bone marrow mesenchymal stem cells is better than those derived from 8 weeks, 12 weeks and 40weeks especially in hypoxia environment.