比色法研究二硫苏糖醇诱导12 nm粒径纳米金的聚集作用

Research on the aggregation of dithiothreitol induced 12 nm gold nanoparticles by colorimetric assay

利用纳米金的比色效应研究了二硫苏糖醇(DTT)对12 nm粒径的纳米金溶液诱导产生的聚集作用。结果表明:DTT诱导聚集纳米金的作用与大分子溶液对胶体溶液的絮凝作用和稳定作用相似;当DTT分子与纳米金粒子的比值达到(1500±500):1时,DTT可迅速桥联纳米金粒子使其聚集,致使溶液发生明显的颜色变化;降低纳米金溶液浓度可有效缓解DTT的聚集作用。为基于纳米金比色反应的检测方法研究提供了一定的理论基础,也对纳米金粒子和DTT在生物化学和分析化学领域的进一步应用具有指导意义。

Gold nanoparticles（ GNP） in size of about 12 nm were usually applied to the fast detection of a variety of biological substances as a kind of color carrier due to its exceptional optical property.The aggregation of dithiothreitol（ DTT） induced 12 nm GNP was studied by the colorimetric assay.The results indicated that the aggregation effect of GNP induced by DTT was consistent with the flocculation and stabilization of macromolecules solution on the colloidal solution; when the ratio of DTT molecules to GNP reached（ 1500 ± 500） ： 1,the GNP solution would change in color quickly because DTT linked GNP together for serious aggregation; and the aggregation could be reduced by diluting the concentration of GNP. The results provided theoretical support for study of numerous detecting methods based on the colorimetric effect of GNP,and gave a guidance for the further application of DTT and GNP in the fields of biochemistry and analytical chemistry.