贵州苗药头花蓼的rbcL序列分析

rbcL Sequence Analysis of Polygonum capitatum in Guizhou

为头花蓼的分子生物学鉴定提供依据,用改良的CTAB法提取贵州不同产地野生和栽培的头花蓼基因组DNA,采用通用引物对不同来源的头花蓼的rbcL序列进行PCR扩增,对扩增产物进行碱基序列测定,运用Clustal X和DNAMAN软件对目的序列进行序列比对和聚类分析。结果表明:改良的CTAB法可有效提取植物总DNA(OD260/OD280为1.1~2.0),rbcL序列长度为979~985bp,目的序列具有高度同源性;测序结果提交NCBI获得GenBank序列号为GP215865-870。rbcL序列信息可作为分子水平鉴定头花蓼的参考依据。

The genomic DNA of wild and cultivated Polygonum capitatum was extracted by CTAB method firstly,secondly rbcLsequences of P.capitatumfrom different sources were amplified by using universal primers,thirdly the base sequence of the amplified products was detected and fourthly the target sequences were compared and clustered by using Clustal X and DNAMAN software to provide a reference for molecular biological identification of P.capitatum.Result：Total DNA（OD260/OD280=1.1～2.0）of P.capitatum can be effectively extracted by CTAB method and the length of rbcL sequence is 979～985 bp.The target sequences have high homologous.The sequencing result is listed as GP215865-870 in GenBank after submitting to NCBI.The rbcLsequence information can be used as a reference basis for molecular biological identification of P.capitatum.