黄桷兰乙醇溶液对小鼠止痒作用的初步研究

Preliminary Study of Antipruritic Effect of Huangjuelan Ethanol Solution on Mice

目的探讨黄桷兰乙醇溶液是否对蚊痒小鼠有治疗作用。方法选择健康成年昆明小鼠60只,雌雄各半,在其耳后注射磷酸组胺建立瘙痒模型。根据体质量随机分组,每组10只,将不同浓度组(高、中、低)的黄桷兰乙醇溶液和其他处理组的药物涂抹于小鼠耳后,观察并记录用药后30 min之内小鼠的搔抓次数和搔抓潜伏期;断头取血,用ELISA法检测血清中IL-6的浓度;取耳后皮肤制作病理切片观察炎症细胞浸润、增生程度;用SPSS 20.0对数据进行统计学分析。结果低浓度(0.02 g/mL)的黄桷兰乙醇溶液可以明显减少搔抓次数(P<0.05),高浓度(0.5 g/mL)的黄桷兰乙醇溶液对炎症细胞的浸润、增生有显著的抑制作用,各组间血清IL-6浓度差异无统计学意义(P>0.05)。结论黄桷兰乙醇溶液对小鼠瘙痒有一定的抑制作用,且低剂量溶液止痒效果好,但尚需在以后的研究中扩大样本量进行进一步研究,进而探讨黄桷兰乙醇溶液的作用机制。

Objective To explore the antipruritic effect of Huangjuelan ethanol solution on the mosquito biting model mice.Methods 60 mice, half male and half female, were selected and 10 mice in each group. Itching model was established through the intradermal injection of histamine phosphate at the back of mouse ear. Different concentrations of Huangjuelan ethanol solution and other treatment were applied on the mouse ear, scratching frequency and scratching latency were observed and recorded within 30 minutes at the wake of intradermal injection of histamine phosphate. All the mice were sacrificed by cutting heads, blood was collected. Serum concentration of IL-6 was detected with ELISA. Skin tissues of mice ear were collected to prepare for the pathological section, inflammatory cell infiltration as well as hyperplasia degree was observed under microscope. SPSS 20.0 was used for statistical analysis. Results The low concentration of Huangjuelan ethanol solution（0.02 g/mL） reduced the scratching frequency significantly（P〈0.01）, while high concentration of Huangjuelan ethanol solution（0.5 g/mL） could significantly inhibit the inflammatory cell infiltration and hyperplasia of mouse ear. No significant difference in serum IL-6 concentration was observed among these groups（P〈0.05）. Conclusion Huangjuelan ethanol solution has a certain inhibitory effect on itching in mice, and low concentration of Huangjuelan solution shows better inhibitory effect. Therefore, it is still necessary to increase the sample size in future studies, and then to explore the mechanism of action of ethanol solution of this plant.